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Ms 275

Manufactured by Enzo Life Sciences
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Sourced in Germany

MS-275 is a histone deacetylase (HDAC) inhibitor, specifically targeting the class I HDAC enzymes. It is a chemical compound used in research applications.

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Lab products found in correlation

Apicidin, by Merck Group (2 mentions) Mocetinostat, by Selleck Chemicals (2 mentions) Mc1568, by Merck Group (2 mentions) Droxinostat, by Selleck Chemicals (2 mentions) Pci 34051, by Cayman Chemical (2 mentions) Calcitriol, by Merck Group (1 mentions) Flavopiridol, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by AppliChem (1 mentions) Trichostatin a tsa, by Merck Group (1 mentions) Goat anti mouse, by Cell Signaling Technology (1 mentions) Hrp linked goat anti rabbit igg, by Cell Signaling Technology (1 mentions) Anti hdac7, by Cell Signaling Technology (1 mentions) Acetyl histone h4, by Merck Group (1 mentions) Nanodrop nd 1000 spectrophotometer, by Thermo Fisher Scientific (1 mentions) Iscript cdna synthesis kit, by Bio-Rad (1 mentions) Lightcycler 96, by Roche (1 mentions) Panobinostat, by Selleck Chemicals (1 mentions) Belinostat, by Selleck Chemicals (1 mentions) Romidepsin, by Selleck Chemicals (1 mentions) Vorinostat, by Merck Group (1 mentions)

6 protocols using ms 275

1

Compound Preparation for Cell Signaling Research

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TSA (Sigma-Aldrich, Schnelldorf, Germany: T1952), MC-1568 (Sigma-Aldrich: M1824), Apicidin (Sigma-Aldrich: A8851), MS-275 (Enzo Life Sciences, Lörrach, Germany: ALX-270-378-M005), PCI-34051 (Cayman Chemical, Ann Arbor, MI, USA: 10444), Mocetinostat (Selleckchem, München, Germany: S1122) and Droxinostat (Selleckchem: S1422) were dissolved in dimethyl sulfoxide (AppliChem, Darmstadt, Germany: A3006). Calcitriol was purchased from Sigma-Aldrich. Human TGFβ1 was purified from platelets according to Werz et al.39 (link)
Ahmad K., Katryniok C., Scholz B., Merkens J., Löscher D., Marschalek R, & Steinhilber D. (2014). Inhibition of class I HDACs abrogates the dominant effect of MLL-AF4 by activation of wild-type MLL. Oncogenesis, 3(11), e127-.
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2

Histone Deacetylase Inhibitor Protocol

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Calcitriol (17936) and the proteasome inhibitor MG132 (Z-Leu-Leu-Leu-al, C2211) were purchased from Sigma-Aldrich, Schnelldorf, Germany. Human TGFβ1 was purified from platelets according to [41 (link)]. TSA (Sigma-Aldrich: T1952), MC-1568 (Sigma-Aldrich: M1824), Apicidin (Sigma-Aldrich: A8851), MS-275 (Enzo Life Sciences, Lörrach, Germany: ALX-270-378-M005), PCI-34051 (Cayman Chemical, Ann Arbor, MI, USA: 10444), Mocetinostat (Selleckchem, München, Germany: S1122), Droxinostat (Selleckchem: S1422) and Flavopiridol (Sigma-Aldrich: F3055) were all dissolved in DMSO (AppliChem, Darmstadt, Germany: A3006). AFF1 ELISA Kit (E98872Hu) was obtained from USCN Life Science, Wuhan, Hubei, PRC.
Ahmad K., Scholz B., Capelo R., Schweighöfer I., Kahnt A.S., Marschalek R, & Steinhilber D. (2015). AF4 and AF4-MLL mediate transcriptional elongation of 5-lipoxygenase mRNA by 1, 25-dihydroxyvitamin D3. Oncotarget, 6(28), 25784-25800.
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3

Histone Acetylation Inhibitor Evaluation

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Trichostatin A (TSA) was purchased from Sigma-Aldrich (Gillingham, UK), CBHA, HAT inhibitor II and Anacardic acid (AA) from Calbiochem (Millipore, Watford, UK), and MS-275 from Enzo Life Sciences (Exeter, UK). Primary rabbit anti-human acetyl-histone H3 and acetyl-histone H4 (Millipore) were used for chromatin immunoprecipitation. Primary mouse anti-human HDAC4 and anti-HDAC7 were used for Western blot and HRP-linked goat anti-rabbit IgG and goat anti-mouse (all from Cell Signalling, Hertfordshire, UK) were used as secondary antibodies. All other reagents were purchased from Sigma-Aldrich unless otherwise stated.
Moores R.C., Brilha S., Schutgens F., Elkington P.T, & Friedland J.S. (2017). Epigenetic Regulation of Matrix Metalloproteinase-1 and -3 Expression in Mycobacterium tuberculosis Infection. Frontiers in Immunology, 8, 602.
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4

Epigenetic Modulation in Brain Tumor Cells

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DAOY, D283 and or UW426 cells were treated with either 5 μmol/L SAHA (Cayman Chemical Company, Ann Arbor, MI) for 6, 12 and 24 hrs, or 2.5 μmol/L MS-275 (Enzo Life Sciences, Farmingdale, NY) for 6, 16 and 24 hrs, or 1.1μM UNC 0638 (IC25 at 48 hrs) for 4 or 12 hrs. Cells were harvested for RNA extraction using Quick RNA kit (Genesee Scientific, San Diego, CA). RNA recovery and purity were determined by NanoDrop Spectrophotometer ND-1000 (Thermo Fischer Scientific, Waltham, MA). cDNA libraries were synthesized using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA) and Q-RT-PCR was performed using Roche Lightcycler 96 (Roche, Indianapolis, IN) according to manufacturer’s instructions. For primers see supplementary data.
Dobson T.H., Hatcher R.J., Swaminathan J., Das C.M., Shaik S., Tao R.H., Milite C., Castellano S., Taylor P.H., Sbardella G, & Gopalakrishnan V. (2017). Regulation of USP37 Expression by REST-associated G9a-dependent Histone Methylation. Molecular cancer research : MCR, 15(8), 1073-1084.
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5

Acquisition of Small Molecule Epigenetic Modulators

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5‐AzadC, NaBut, VPA, and SAHA (vorinostat) were purchased from Sigma Aldrich. MS‐275 was purchased from Enzo Life Sciences. Belinostat, panobinostat, and romidepsin were purchased from Selleckchem.
Bouchat S., Delacourt N., Kula A., Darcis G., Van Driessche B., Corazza F., Gatot J., Melard A., Vanhulle C., Kabeya K., Pardons M., Avettand‐Fenoel V., Clumeck N., De Wit S., Rohr O., Rouzioux C, & Van Lint C. (2015). Sequential treatment with 5‐aza‐2′‐deoxycytidine and deacetylase inhibitors reactivates HIV‐1. EMBO Molecular Medicine, 8(2), 117-138.
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6

Radiosensitization Agents Evaluation

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Bafilomycin-A1, 5-Aza-2′-deoxycytidine (5-Aza), and MS-275 (Enzo Life Science, Farmingdale, NY, USA) were purchased from Sigma (St. Louis, MO, USA). For irradiation, a 6 MV photon beam generated by a linear accelerator (CLINAC 600 C; Varian, Palo Alto, CA, USA) at a dose rate of 2 Gy/min was used.
Lee M., Nam H.Y., Kang H.B., Lee W.H., Lee G.H., Sung G.J., Han M.W., Cho K.J., Chang E.J., Choi K.C., Kim S.W, & Kim S.Y. (2021). Epigenetic regulation of p62/SQSTM1 overcomes the radioresistance of head and neck cancer cells via autophagy-dependent senescence induction. Cell Death & Disease, 12(3), 250.
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