Rabbit anti atf 6α

The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 2′,7′-dichlorofluorescin diacetate (DCFDA) were purchased from Sigma-Aldrich. The following primary antibodies used were: rabbit polyclonal anti-β-actin, goat anti-PTPase, 1B, rabbit anti-BIP, rabbit anti-GADD 153, rabbit anti-XBP-1, rabbit anti-ATF-6α, rabbit anti-eIF2α, rabbit anti-SOD2, rabbit anti-MMP-2, rabbit anti-TIMP-2, rabbit anti-IL6 and rabbit anti-IL8 (all from Santa Cruz Biotechnology). The secondary antibodies were horseradish peroxidase (HRP)-conjugated anti-rabbit, HRP-conjugated anti-mouse, HRP-conjugated anti-goat (Santa Cruz Biotechnology) and anti-mouse IgG, FITC conjugate from Invitrogen. Laemmli Sample Buffer 2x (SX2) was acquired from Serva and enhanced chemiluminescence reagent Supersignal Ultra from Pierce Biotechnology.

Monoclonal mouse anti-p21 (1:200), polyclonal rabbit anti-PERK (phospho T981) (1:200), anti-PERK (1:200), and rabbit anti-ATF6α (1:200) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); monoclonal mouse anti-CHOP antibody (1:1000) was obtained from Cell Signaling Technology (Danvers, MA, USA). Monoclonal rabbit anti-histone H3 (phospho S10) (1:1000), monoclonal rabbit anti-GCN2 (1:1000), and monoclonal rabbit anti-GCN2 (phospho T899) (1:500) antibodies were obtained from Abcam (Cambridge, UK). Monoclonal mouse anti-phospho-Histone H2A.X (Ser139) (1:50) antibody was purchased from Millipore (Billerica, MA, USA). Finally, polyclonal rabbit anti-actin (1:2000) antibody was obtained from Sigma-Aldrich (St. Louis, MO, USA).