Recombinant human adiponectin

Manufactured by R&D Systems

Sourced in United States

Recombinant human adiponectin is a protein produced in a laboratory setting. It is a member of the adipokine family and is involved in the regulation of glucose and lipid metabolism.

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Close spelling variants of this product

Recombinant human adiponectin/aCrp30 Adiponectin

3 protocols using recombinant human adiponectin

Adiponectin Signaling Pathway Characterization

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Anti-mouse and anti-rabbit horseradish peroxidase-conjugated IgG, rabbit polyclonal antibodies against AdipoR1, AdipoR2, β-actin, phosphorylated-p85 (p-p85), p85, phosphorylated-Akt (p-Akt), Akt, phosphorylated-mTOR (p-mTOR), and mTOR were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). HIF-1α, VEGF-A, and CD31 antibodies were purchased from Abcam (Cambridge, MA, USA). Recombinant human adiponectin and VEGF-A were purchased from R&D Systems (Minneapolis, MN, USA). pHRE-luciferase construct was provided from Dr. W.M. Fu (National Taiwan University, Taiwan). The pSV-β-galactosidase vector and luciferase assay kit were purchased from Promega (Madison, WI, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Lee H.P., Lin C.Y., Shih J.S., Fong Y.C., Wang S.W., Li T.M, & Tang C.H. (2015). Adiponectin promotes VEGF-A-dependent angiogenesis in human chondrosarcoma through PI3K, Akt, mTOR, and HIF-α pathway. Oncotarget, 6(34), 36746-36761.

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Cell Culture Reagents and Materials

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Dulbecco's Modified Eagle's Medium and Ham F-12 Nutrient Mix (DMEM/F12), Roswell Park Memorial Institute (RPMI) medium, progesterone, E2, penicillin, streptomycin, DNase type I, EDTA, leupeptin, aprotinin, 4-(2-aminoethyl)-benzene-sulfonyl fluoride (AEBSF), sodium fluoride and bovine serum albumin (BSA) were purchased from Sigma Chemical Co. Fetal calf serum (FCS) was purchased from Gibco (Invitrogen). Recombinant human adiponectin was provided by R&D Systems Europe Ltd. Superscript III RNase H-RT and primers were from Invitrogen, and RNase inhibitor was obtained from AMRESCO (Solon, OH, USA). Nucleospin RNA II kit was obtained from Machery-Nagel (Düren, Germany). Trypsin was provided by Difco Laboratories (Detroit, MI, USA). Matrigel was obtained from BD Biosciences (Le Pont-de-Claix, France) and collagenase A was from Boehringer (Mannheim, Germany). The suppliers of the various antibodies used are described in the corresponding paragraphs below.

Duval F., Dos Santos E., Moindjie H., Serazin V., Swierkowski-Blanchard N., Vialard F, & Dieudonné M.N. (2017). Adiponectin limits differentiation and trophoblast invasion in human endometrial cells. Journal of molecular endocrinology, 59(3).

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Metabolic Biomarkers in Pre-Surgery Fasting

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Pre-surgery fasting (at least 4 hours) plasma levels of glucose and serum insulin, adiponectin, leptin and fetuin-A were determined following blood collection in standard serum tubes and centrifuged at 3,000rpm for 15min at 5°C. Serum (3-5mls) was stored at -80°C before transfer for analysis at the Medical Re search Laboratory, University of Aarhus, Denmark. The assay techniques have been described elsewhere. 20 Briefly, glucose concentrations were analyzed by the glucose oxidase method, Beckman Instruments, CA, USA; insulin by a two-site immunospecific Enzyme-Linked ImmunoSorbent Assay (ELISA), Dako, Denmark; adiponectin by an in-house Time-Resolved ImmunoFluoroMetric Assay (TR-IFMA) based on two monoclonal antibodies and recombinant human adiponectin (R&D Systems, Abingdon, UK); leptin by a validated in-house TR-IFMA based on two commercial monoclonal antibodies and commercial recombinant human leptin as the standard, R&D Systems, Abingdon, UK. Fetuin-A levels were determined by a commercial Quantikine ELISA from R&D Systems in accordance with instructions from the manufacturer. To estimate insulin resistance, we derived a HOmeostasis Model Assessment of Insulin Resistance (HOMA-IR) value for each patient. 21 HOMA-IR has good correlation with the euglycaemic clamp (r = 0.88) and has been validated in healthy subjects. 22

Parkin E., O'Reilly D.A., Plumb A.A., Manoharan P., Rao M., Coe P., Frystyk J., Ammori B., de Liguori Carino N., Deshpande R., Sherlock D.J, & Renehan A.G. (2015). Digital histology quantification of intra-hepatic fat in patients undergoing liver resection. European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology, 41(8).

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