Skin fibroblasts derived from healthy donors (GM02036 from 11-year female, GM05659 from 1-year male, GM05756 from 2-month male, and GM08398 from 8-year male) were obtained from Coriell Institute for Medical Research (Camden, NJ). Fibroblasts were cultured in DMEM (Life Technologies 11195–073) supplemented with 10% fetal bovine serum (FBS), 100 units/ml penicillin and 100 μg/ml streptomycin (Life technologies 10378–016) in a humidified incubator with 5% CO2 and 20% O2 at 37 °C. Cells were routinely trypsinized (0.05% trypsin/EDTA) and passaged until reprogramming. Human iPSCs were maintained on Matrigel (Corning, #354230) in the chemically-defined Essential 8 medium (Life Technologies, #A1517001) and passaged using 0.5 mM EDTA/DPBS, as described previously (Beers et al., 2012 (link)) (Table 1 ).
Gm02036
Manufactured by Coriell Institute for Medical Research
GM02036 is a cell line derived from a human male. It is a primary skin fibroblast cell line that can be used for various research purposes. The core function of this cell line is to provide a controlled and standardized cellular model for scientific investigations.
Automatically generated - may contain errors
Lab products found in correlation
Essential 8 medium, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Matrigel, by Corning (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Gm05659, by Coriell Institute for Medical Research (2 mentions)
Gm08398, by Coriell Institute for Medical Research (2 mentions)
Gm05756, by Coriell Institute for Medical Research (2 mentions)
2 protocols using gm02036
1
Generating Human iPSCs from Skin Fibroblasts
2
Generating Human iPSCs from Skin Fibroblasts
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Skin fibroblasts derived from healthy donors (GM02036 from 11-year female, GM05659 from 1-year male, GM05756 from 2-month male, and GM08398 from 8-year male) were obtained from Coriell Institute for Medical Research (Camden, NJ). Fibroblasts were cultured in DMEM (Life Technologies 11195–073) supplemented with 10% fetal bovine serum (FBS), 100 units/ml penicillin and 100 μg/ml streptomycin (Life technologies 10378–016) in a humidified incubator with 5% CO2 and 20% O2 at 37 °C. Cells were routinely trypsinized (0.05% trypsin/EDTA) and passaged until reprogramming. Human iPSCs were maintained on Matrigel (Corning, #354230) in the chemically-defined Essential 8 medium (Life Technologies, #A1517001) and passaged using 0.5 mM EDTA/DPBS, as described previously (Beers et al., 2012 (link)) (Table 1 ).
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