Western blot assays were performed as previously described37 (link). The primary antibodies used in our study were as follows: PEAK1 (Santa Cruz Biotechnology, USA); PEAK1/NKF3, E-cadherin, N-cadherin, MMP2, and MMP9 (Abcam, UK); p-AKT, total AKT, p-ERK1/2, total ERK1/2, p-JNK and total JNK (Invitrogen, USA); p-Rac1, total Rac1, p-JAK1, total JAK1, p-JAK2, total JAK2, Twist1, Twist2, Snail1, Snail2, Zeb1, and Zeb2 (OriGene, USA); and GAPDH (Immunology Consultants Laboratory, USA). Immunoprecipitation was performed exactly as previously described39 (link).
Total akt
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
The Total AKT Assay Kit is a quantitative sandwich immunoassay designed to measure the total AKT protein levels in cell and tissue lysates. AKT, also known as Protein Kinase B, is a serine/threonine-specific protein kinase that plays a key role in the PI3K/AKT signaling pathway involved in cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription, and cell migration.
Automatically generated - may contain errors
Lab products found in correlation
Total erk1 2, by Thermo Fisher Scientific (3 mentions)
Mmp 9, by Abcam (2 mentions)
E cadherin, by Abcam (2 mentions)
Phospho akt, by Thermo Fisher Scientific (2 mentions)
Phospho erk1 2, by Thermo Fisher Scientific (2 mentions)
Snail1, by OriGene (1 mentions)
Perk1 2, by Thermo Fisher Scientific (1 mentions)
P akt, by Thermo Fisher Scientific (1 mentions)
N cadherin, by Abcam (1 mentions)
Vimentin, by Abcam (1 mentions)
Phospho nf κb p65, by Abcam (1 mentions)
Total nf κb p65, by Abcam (1 mentions)
Phospho iκbα, by Abcam (1 mentions)
Vascular endothelial growth factor (vegf), by Abcam (1 mentions)
3 protocols using total akt
1
Western Blotting and Immunoprecipitation Assays
2
Comprehensive Protein Expression Analysis
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Western blot analysis was performed as previously described [14 (link)]. The following commercial antibodies were used in this study: Gab2 (OriGene Technologies, USA), E-cadherin, vimentin, MMP7 and MMP9 (Abcam, UK), phospho-AKT, total AKT, phospho-ERK1/2 and total ERK1/2 (Invitrogen, USA), GAPDH and β-actin (Immunology Consultants Laboratory, USA).
3
Western Blot Analysis of Signaling Proteins
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Western blot analysis was performed as previous described [19 (link)]. The following commercial antibodies were used in this study: VEGF, EGFR, phospho-c-Myc, total c-Myc, phospho-NF-κB/p65, total NF-κB/p65, phospho-IκBα and total IκBα (Abcam, UK), phospho-AKT, total AKT, phospho-STAT3, total STAT3, phospho-ERK1/2 and total ERK1/2 (Invitrogen, USA), GAPDH and β-actin (Immunology Consultants Laboratory, USA).
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