Rabbit anti trka

The Müller cells were washed with PBS (1X; 135 mM NaCl, 4.7 mM KCl, 10 mM Na₂HPO₄, 2 mM NaH₂PO₄, pH 7.4) twice and fixed for 20 min in 4% paraformaldehyde at room temperature. Then, the cells were blocked with 3% BSA in PBS for 1 h and incubated with 1:100 mouse monoclonal anti-GS antibody (Abcam, Cambridge, UK) and 1:100 rabbit anti-TrkA (Cell Signaling Technology, Beverly, MA) antibody overnight at 4 °C. After washing with PBS/0.1% Tween-20 three times, the cells were incubated with 1:200 Alexa 555-conjugated donkey ant-mouse/Alexa 488-conjugated donkey anti-rabbit antibodies (Cell Signaling Technology) at room temperature for 1 h. The nuclei were stained with 1:1,000 4’,6-diamidino-2-phenylindole (DAPI) for 5 min, and the cells were analyzed under a confocal microscope (LSM 510 META; Carl Zeiss, Oberkochen, Germany). Fluorescence pictures were taken with identical exposure settings. For the negative control, the cells were stained without primary antibodies and showed no signals.