Protein kinase inhibitor was purchased from Calbiochem; trypsin was purchased from Promega; acetonitrile and ultrapure water (H2O) were purchased from Fisher Chemical; trifluoroacetic acid, iodoacetamide, dithiothreitol, urea, and triethylammonium bicarbonate (TEAB) were purchased from Sigma; formic acid purchased from Fluka. Zhishi Rhubarb Soup (ZRS), a Chinese herbal regimen, was purchased from the Department of Pharmacy, Nanjing Chinese Medicine Hospital Affiliated to Nanjing Universityof Traditional Chinese Medicine, decocted according to the conventional method, concentrated to 2.5 g/ml and stored at 4 °C for later use.
Ultrapure water h2o
Manufactured by Thermo Fisher Scientific
Ultrapure water (H2O) is a laboratory-grade water product that undergoes extensive purification to remove impurities and contaminants. It is characterized by its extremely low levels of dissolved ions, organic compounds, and particulates, resulting in a high-purity water source suitable for sensitive applications in scientific research and analysis.
Automatically generated - may contain errors
2 protocols using ultrapure water h2o
1
Herbal Extract Identification Protocol
2
Bioactive Compounds Extraction and Analysis
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Protein kinase inhibitor was purchased from Calbiochem; trypsin was purchased from Promega; acetonitrile, Ultra-pure water (H2O) from Fisher Chemical; tri uoroacetic acid , formic acid , Fluka, iodoacetamide, dithiothreitol , urea , Triethylammonium bicarbonate TEAB from Sigma. Zhishi Rhubarb Soup (ZRS), Chinese herbal regimen was Purchased from the Department of Pharmacy, Nanjing University of Traditional Chinese Medicine A liated to Nanjing University of Traditional Chinese Medicine, decocted according to the conventional method, concentrated to 2.5g/ml and stored at 4℃ for later use. Zhishi Rhubarb Soup is consisted of cooked rhubarb 10g, citrus aurantium 10g, magnolia root 10g, scutellaria 10g, woody 3g, licorice 3g.
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