N-ethylmaleimide (NEM) was purchased from Pierce (Rockford, IL). Duo-Lux chemiluminescent / fluorescent substrate was purchased from Vector Labs (Burlingame, CA). Anti-peroxiredoxin-2 antibody was purchased from Abcam (Cambridge, MA). Goat anti-rabbit IgG antibody conjugated to alkaline phosphatase was purchased from Millipore (Billerica, MA). Nitrocellulose (162-0112, 0.2μm pore size) was purchased from Bio-Rad (Hercules, CA). Percoll was purchased from GE healthcare life science (Pittsburgh, PA). All other materials were purchased from Sigma–Aldrich (St Louis, MO).
Anti peroxiredoxin 2 antibody
Manufactured by Abcam
Anti-peroxiredoxin-2 antibody is a laboratory product used for the detection and analysis of peroxiredoxin-2 protein in various samples. Peroxiredoxin-2 is an antioxidant enzyme involved in the regulation of cellular redox homeostasis.
Automatically generated - may contain errors
Lab products found in correlation
Percoll, by GE Healthcare (1 mentions)
Goat anti rabbit igg antibody conjugated to alkaline phosphatase, by Merck Group (1 mentions)
Duolux chemiluminescent fluorescent substrate, by Vector Laboratories (1 mentions)
Goat anti mouse igg peroxidase, by Merck Group (1 mentions)
Anti vimentin antibody, by Thermo Fisher Scientific (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti peroxiredoxin 2 antibody
1
Protein Detection Using Chemiluminescence
2
Antibody Dilutions for Western Blot Analysis
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Antibodies were purchased from the following sources: anti-Peroxiredoxin 2 antibody used at 1/1000 dilution (abcam), anti-Galectin 1 antibody used at 1/1000 dilution (Thermo scientific), anti-Vimentin antibody used at 1/1000 dilution (Thermo scientific), anti-Glutathione antibody [D8] used at 1/1000 dilution or 1/2000 (abcam), anti-glutathione transferase Omega 1 used at 1/1000 dilution (abcam) and anti-GAPDH used at 1/1000 dilution (Cell Signaling Technology). The secondary antibodies used were a goat anti-rabbit IgG-HRP used at 1/2000 or 1/5000 dilution (Santa Cruz Biotechnology) and a goat anti-mouse IgG peroxidase used at 1/5000 or 1/12000 dilution (Sigma-Aldrich).
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