prepared by using deionized water with a resistivity of 18.2 Ω
cm–1 from a Millipore Milli-Q water purification
system (Millipore). Gluten from wheat was purchase from Sigma-Aldrich.
1,2-Dimethyl imidazole (98%), 1-fuloro pentane, methanol (99%), propanol
(99%), pentanol (99%), heptanol (98%), nonanol (98%), and dodecanol
(98%) were purchased from ACOS; dichloromethane (99%) was purchased
from Tedia (98% purity); acetonitrile (99%), ether (99%), fetal bovine
serum (FBS), Gluten from wheat, methanesulfonic acid (99), methane
sulfonic chloride (99%), thionyl chloride (98%), silver nitride (95%),
silver dihydrogen phosphate (99%), and silver bisulfate (99%) were
purchased from Sigma-Aldrich; antigliadin mouse IgG 2F (1 mg/mL) was
purchased from Antaimmu; antigliadin human IgA 3B7 (1 mg/mL) and a
gliadin solution (1 mg/mL) were purchased from Leadgene; HRP was present
in the MagicLink HRP antibody conjugation kit; 3,3′,5,5′-tetramethylbenzidine
(TMB) and hydrogen peroxide (H2O2) reagent were
purchased from TCI; and the Wheat/Gluten (Gliadin) ELISA kit was purchased
from Crystal Chem (AOAC no. 011804). DEMD and XTT reagents were purchased
from Thermo Fisher.
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is given in