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Gluten from wheat

Manufactured by Merck Group
Sourced in Australia

Gluten from wheat is a protein complex extracted from wheat. It is a key component in the production and processing of various food and pharmaceutical products. The core function of gluten is to provide structure, texture, and binding properties to these products.

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2 protocols using gluten from wheat

1

Synthesis and Characterization of Imidazolium-based Ionic Liquids

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All solutions and samples were
prepared by using deionized water with a resistivity of 18.2 Ω
cm–1 from a Millipore Milli-Q water purification
system (Millipore). Gluten from wheat was purchase from Sigma-Aldrich.
1,2-Dimethyl imidazole (98%), 1-fuloro pentane, methanol (99%), propanol
(99%), pentanol (99%), heptanol (98%), nonanol (98%), and dodecanol
(98%) were purchased from ACOS; dichloromethane (99%) was purchased
from Tedia (98% purity); acetonitrile (99%), ether (99%), fetal bovine
serum (FBS), Gluten from wheat, methanesulfonic acid (99), methane
sulfonic chloride (99%), thionyl chloride (98%), silver nitride (95%),
silver dihydrogen phosphate (99%), and silver bisulfate (99%) were
purchased from Sigma-Aldrich; antigliadin mouse IgG 2F (1 mg/mL) was
purchased from Antaimmu; antigliadin human IgA 3B7 (1 mg/mL) and a
gliadin solution (1 mg/mL) were purchased from Leadgene; HRP was present
in the MagicLink HRP antibody conjugation kit; 3,3′,5,5′-tetramethylbenzidine
(TMB) and hydrogen peroxide (H2O2) reagent were
purchased from TCI; and the Wheat/Gluten (Gliadin) ELISA kit was purchased
from Crystal Chem (AOAC no. 011804). DEMD and XTT reagents were purchased
from Thermo Fisher.
IL synthesis is given in the Supporting Information, and the product yield
is given in Table S1.
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2

Wheat Gluten Extraction and Characterization

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Gluten from wheat, ammonium persulfate (APS), and tris(2,2-bipyridyl)dichlororuthenium(II) hexahydrate (Ru(BPY)3) were procured from Sigma-Aldrich, Sydney, Australia. Keratin powder was purchased from Spectrum Chemicals, Australia. Isopropyl alcohol and ethanol were obtained from Chem-Supply, Adelaide, Australia.
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