Blood glucose levels were measured using OneTouch Verio (LifeScan Europe, Johnson & Johnson, Sug, Switzerland). OneTouch Verio uses a finger prick to collect a blood sample via test strips which are accurate and precise over a wide range of patients and environmental and pharmacologic conditions [20 (link)]. After a 10–12 h overnight fast period, two fasting blood drops were collected 5 min apart. If the difference between the two fasting blood glucose levels was more than 0.2 nmol/L, a third blood drop was collected. Then, participants were randomly given a test beverage with either a low (isomaltulose) or a high (sucrose) glycemic index. Then, 50 ± 0.01 g of sucrose (Kristalsuiker, Delhaize, Brussels, Belgium) or isomaltulose (Palatinose™, provided by BENEO, Brussels, Belgium) was measured on a calibrated electronic laboratory scale (AX124, Sartorius, Goettingen, Germany) and dissolved in 250 ± 0.1 mL of plain drinking water measured out with volumetric laboratory equipment. Afterward, blood glucose levels were collected at 15, 30, 45, 60, 90, and 120 min after consumption of the beverages. The first two drops of blood were discarded, and the third drop was used for testing. The same procedure was applied during the first and second assessment sessions.
Ax124
Manufactured by Sartorius
Sourced in Germany
The AX124 is a precision balance from Sartorius, designed to provide accurate weighing results. It features a weighing capacity of up to 120 grams and a readability of 0.1 milligrams. The balance is suitable for a variety of laboratory applications requiring high-precision measurements.
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Lab products found in correlation
3 protocols using ax124
1
Glycemic Response to Isomaltulose and Sucrose
2
Measuring Weights and Anogenital Distance
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Body weights of C- and R-females/fetuses/offspring were measured with a portable balance (EK-3000i) (A&D, Tokyo, Japan), and testis weights of C- and R-offspring were measured with an analytical electronic balance (AX124) (Sartorius AG, Göttingen, Germany). AGD was measured with a Digimatic Caliper (Mitsutoyo, Kawasaki, Japan) under a EZ4 D microscope (Leica, Wetzlar, Germany). The AGD index (AGDI) was also calculated by diving the AGD by the cube root of body weight [17 (link)], to allow for comparison of AGDs between C-fetuses and R-fetuses with different body size.
3
Cadmium Distribution in Maize Leaves
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We determined Cd concentrations in one-centimetre segments sampled along the maize leaf growth zone (i.e. 10 centimetres in total) and included a blade segment (middle of the remaining blade). Fresh weight of the sampled leaf segments was measured (AX124, Sartorius, Göttingen, Germany), after which they were oven-dried at 60 °C for 48 to 72 hours.
Hereafter, segments from the same position and treatment were pooled (2-3 segments per pool). Sample digestion was performed by an overnight predigestion in aqua regia (1:3 nitric acid and hydrochloric acid), followed by 20 minutes high pressure high temperature digestion (Discover SP-D, CEM, Matthews, NC, USA), allowing the samples to boil at 200 °C. The samples were then diluted 40 times with trace metal grade ultrapure water, after which the Cd concentration was measured with high resolution inductively coupled plasma mass spectrometry (Element XR, Thermo Scientific, Bremen, Germany). We used blanks to correct for background trace metals and Rye grass European Reference Material CD281 samples as a reference.
Hereafter, segments from the same position and treatment were pooled (2-3 segments per pool). Sample digestion was performed by an overnight predigestion in aqua regia (1:3 nitric acid and hydrochloric acid), followed by 20 minutes high pressure high temperature digestion (Discover SP-D, CEM, Matthews, NC, USA), allowing the samples to boil at 200 °C. The samples were then diluted 40 times with trace metal grade ultrapure water, after which the Cd concentration was measured with high resolution inductively coupled plasma mass spectrometry (Element XR, Thermo Scientific, Bremen, Germany). We used blanks to correct for background trace metals and Rye grass European Reference Material CD281 samples as a reference.
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