The samples fixed with FAA were subjected to the following treatments: (a) a part of the samples was embedded in paraffin [66 (link)]; (b) another part of the samples was embedded in LR-White® Hard Grade resin (London Resin Company, London, UK), according to Barreno et al. [30 ]; (c) finally, other samples were saved for observation using a low-temperature scanning microscope (LTSEM).
All the sections were observed with an OLYMPUS Provis AX 70 (Olympus, Hamburg, Germany) light microscope equipped with epifluorescence equipment. The observations with fluorescence and autofluorescence were performed with a UMWU fluorescence cube (excitation filter: 330–385 nm; barrier filter: 420 nm; dichroic mirror: 400 nm). All the images were photographed with a Lumenera Infinity 2-3C digital CCD colour camera (2080 × 1536 resolution, Lumenera, Ottawa, ON, Canada), captured with the Infinity Analyze® 7 v program. 7.1.0, and subsequently processed with the Photoshop CC® 2018 program, at the Jardí Botànic of the University of Valencia.
All the sections were observed with an OLYMPUS Provis AX 70 (Olympus, Hamburg, Germany) light microscope equipped with epifluorescence equipment. The observations with fluorescence and autofluorescence were performed with a UMWU fluorescence cube (excitation filter: 330–385 nm; barrier filter: 420 nm; dichroic mirror: 400 nm). All the images were photographed with a Lumenera Infinity 2-3C digital CCD colour camera (2080 × 1536 resolution, Lumenera, Ottawa, ON, Canada), captured with the Infinity Analyze® 7 v program. 7.1.0, and subsequently processed with the Photoshop CC® 2018 program, at the Jardí Botànic of the University of Valencia.