B. velezensis (DH82 strain; GenBank: MK203035) was isolated from the sea water samples of the Western Pacific Yap trench at the depth of 6000 m, and was kindly offered by the Third Institute of Oceanography (Xiamen, China). P. aerugnosa wild-type strain was isolated from the water lines of drinking fountains in this study. The AHL reporter operon of LuxR-PluxI-lacO-RFP was provided by Xiamen University (Xiamen, China), and was conducted on pET28a in E. coli BL21 to detect the level of AHLs. N-Acyl-L-homoserine lactone hydrolase (PDB: 3DHB) from B. thuringiensis (GenBank: AY943832), named AiiA3DHB, was used as the positive control to analyze the QQ activity of AiiADH82. All of the bacterial strains were cultured in a Luria–Bertani (LB) medium.
E. coli DH5α and BL21 (DE3) competent cells were purchased from Transgen (Beijing, China). The restriction endonucleases and ligation enzymes were purchased from Takara Biotechnology (Dalian, China). The plasmid miniprep kit (Cat. GMK5999) and gel extraction kit (Cat. D2500-02) were purchased from Promega. N-(β-Ketocaproyl)-DL-homoserine lactone (C6-(L)-HSL, Cat. K3255) and (3-Oxododecanoyl)-L-homoserine lactone (3-oxo-C12-(L)-HSL, Cat. 09139) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
E. coli DH5α and BL21 (DE3) competent cells were purchased from Transgen (Beijing, China). The restriction endonucleases and ligation enzymes were purchased from Takara Biotechnology (Dalian, China). The plasmid miniprep kit (Cat. GMK5999) and gel extraction kit (Cat. D2500-02) were purchased from Promega. N-(β-Ketocaproyl)-DL-homoserine lactone (C6-(L)-HSL, Cat. K3255) and (3-Oxododecanoyl)-L-homoserine lactone (3-oxo-C12-(L)-HSL, Cat. 09139) were purchased from Sigma-Aldrich (St. Louis, MO, USA).