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Anti il 12p40 clone c17.8

Manufactured by BioLegend
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Anti-IL-12p40 (clone C17.8) is a mouse monoclonal antibody that binds to the p40 subunit of interleukin-12 (IL-12). It is commonly used in research applications as a tool to study the functions and signaling pathways of IL-12.

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Lab products found in correlation

Recombinant murine il 12p70, by Thermo Fisher Scientific (1 mentions)

2 protocols using anti il 12p40 clone c17.8

1

Modulating IL-12 in Y. pseudotuberculosis MLN

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At the start of Y. pseudotuberculosis stimulation of MLN cell suspensions, cultures were treated with 10 μg/ml anti-IL-12p40 (clone C17.8; BioLegend) for neutralization. In other conditions, recombinant murine IL-12p70 (Peprotech) was added at the start of Y. pseudotuberculosis stimulation of MLN cell suspensions at 2, 10, or 50 ng/ml.
Chu T.H., Khairallah C., Shieh J., Cho R., Qiu Z., Zhang Y., Eskiocak O., Thanassi D.G., Kaplan M.H., Beyaz S., Yang V.W., Bliska J.B, & Sheridan B.S. (2021). γδ T cell IFNγ production is directly subverted by Yersinia pseudotuberculosis outer protein YopJ in mice and humans. PLoS Pathogens, 17(12), e1010103.
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2

Anti-IL-12p40 Treatment in Yop Infection

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On the day of foodborne WT or YopJC172AY. pseudotuberculosis infection and on day 2, 4, and 6 after infection, mice were treated with 0.2 mg of anti-IL-12p40 (clone C17.8; BioLegend) by i.p. injection. Blood was collected via tail vein on day 2, 4, and 6 after infection. Blood was incubated at ambient temperature for 30 minutes before being spun down at 1500G for 10 minutes at 4°C. Serum was isolated and analyzed for IL-12p70 with the BioLegend ELISA MAX Deluxe Set Mouse IL-12 (p70) kit.
Chu T.H., Khairallah C., Shieh J., Cho R., Qiu Z., Zhang Y., Eskiocak O., Thanassi D.G., Kaplan M.H., Beyaz S., Yang V.W., Bliska J.B, & Sheridan B.S. (2021). γδ T cell IFNγ production is directly subverted by Yersinia pseudotuberculosis outer protein YopJ in mice and humans. PLoS Pathogens, 17(12), e1010103.
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