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5 protocols using polylactic acid

1

Synthesis and Characterization of Polymer Composites

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Chloroplatinic
acid (8 wt %), ethylene glycol, l-(+)-lactic acid, melamine,
polypropylene, polystyrene (pellets, Mw 35000), and polystyrene-block-polybutadiene were
purchased from Sigma-Aldrich. Low-density polyethylene
(powder, 300 μm), poly(ethylene terepththalate) (powder, 300
μm), poly(lactic acid) (pellets, 3 mm), and polyurethane (foam,
10 × 100 × 100 mm3, density 0.08 g cm–3) were obtained from Goodfellow Cambridge Ltd. polypropylene, polystyrene,
polystyrene-block-polybutadiene, poly(lactic acid),
and polyurethane were frozen with liquid N2 and then ground
in a coffee grinder to powders prior to use. NaOD (40 wt % in D2O), KOH (semiconductor grade), KSCN, maleic acid, nickel(II)
chloride hexahydrate, sodium hypophosphite monohydrate, soybean oil,
and terephthalic acid were purchased from Fischer Scientific. D2O (99.96 atom % D) was obtained from Euriso-Top, and TiO2 nanoparticles (P25, 10–30 nm) were purchased from
SkySpring Nanomaterials, Inc. A plastic water bottle (still Scottish
mountain water) was purchased from Marks and Spencer Simply Food,
dried, and ground by using a coffee grinder into pieces ≤0.5
cm2. Polyester microfibers (Homescapes Super Microfibre)
were used as received.
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2

Fabrication of PLA-Mg Alloy Particles

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Polylactic acid (PLA) particles with a nominal granule size of 3–5 mm, a melting point around 170 °C, and an average molecular weight of approximately 193,300 g/mol, were purchased from Goodfellow Cambridge Limited (Cambridge, UK). The density and Melt flow rate (MFR) of the PLA are 1.24 g/cm3 and 8, respectively. Mg alloy (WE43, purity 99.95%) powder with an average diameter of 35–50 μm was provided by Nanografi Nanotechnology (Ankara, Turkey) (SEM images shown in Figure 1). Chloroform (Boiling point: 59.5–60.5 °C, density: 1.48 kg/L) was used as a PLA solvent supplied by VWR Chemicals (Hannover, Germany).
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3

Fabrication of PLA-Mg Composite Materials

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Polylactic acid (PLA) granules with a nominal size of 3–5 mm, a melting point of around 170 °C, and an average molecular weight of approximately 68,000 g/mol, were purchased from Goodfellow Cambridge Limited (Cambridge, England). Mg alloy (AZ61, purity 99.95%) powder with an average diameter of 100 μm was provided by Nanografi Nanotechnology (Ankara, Turkey). Chloroform (boiling point: 59.5–60.5 °C, density: 1.48 kg/L) was used as a PLA solvent supplied by VWR Chemicals, Darmstadt, Germany.
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4

Fabrication of PLA-Mg Composite Materials

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Polylactic acid (PLA) granules with a nominal size of 3–5 mm, a melting point of around 170 °C, and an average molecular weight of approximately 68,000 g/mol, were purchased from Goodfellow Cambridge Limited (Cambridge, England). Mg alloy (AZ61, purity 99.95%) powder with an average diameter of 100 μm was provided by Nanografi Nanotechnology (Ankara, Turkey). Chloroform (boiling point: 59.5–60.5 °C, density: 1.48 kg/L) was used as a PLA solvent supplied by VWR Chemicals, Darmstadt, Germany.
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5

Antiviral Polylactic Acid Coatings

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Polylactic acid (PLA) (Goodfellow., LTD, England) was used as a primary coating substance. Chloroform (Sigma Aldrich, 99.5%) was used as a solvent as it yielded the highest solubility. A magnetic bar was put into a 500 mL beaker, covered with aluminum foil, and autoclaved at 121℃ for 15 min. PLA granules were mixed with Chloroform for 1 to 2 h to reach a concentration of 7.6% (w/v). Mixing was stopped when it was visually confirmed that the PLA particles were completely melted.
Caffeic acid and vanillin were used as antiviral substances. Caffeic acid (108.1 mg) and vanillin (152.15 mg) were dissolved in 10 mL of ethanol to prepare 6,000 and 10,000 µmol/mL solutions. Afterward, a serial dilution was performed to prepare the corresponding 600 and 1000 µmol/mL solutions. Finally, 10 mL of this solution was mixed with 90 mL of the PLA-coating solution to adjust the total volume to 100 mL, stirred vigorously for 1 to 2 h, and 10 mL was poured into stainless steel square frames. The samples were dried at 50 to 60℃ for 2 to 3 days to attain thermodynamic properties.
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