We analyzed the target analytes using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS, ACQUITY, TQD, Waters, Milford, MA, USA), with electrospray in negative ion mode [ES(-)]. To separate the analytes, an ACQUITY BEH C18 column (2.1 × 50 mm; 1.7 m, Waters, USA) was used, and an ACQUITY BEH C18 precolumn (2.1 × 30 mm; 1.7 m, Waters, USA) was inserted between the injector and the solvent mixer to retain any PFAAs contamination from the system. As mobile phase solvents, we used 0.1% formic acid in water (A) and 0.1% formic acid in ACN, with a flow rate of 450 L/min and an injection volume of 10 L. The gradient started at 65% A, decreased to 0% A after 3.4 min and returned to 65% A at 4.7 min. The analytes were identified and quantified using multiple reaction monitoring (MRM) of two diagnostic transitions per analyte. MRM transitions, cone voltages and collision energy of each target analyte, including the ISTDs, are displayed in Table S2 and were validated as described by Groffen et al. [58] .
Acquity beh c18 pre column
Manufactured by Waters Corporation
Sourced in United States
The ACQUITY BEH C18 pre-column is a component of the ACQUITY UPLC system from Waters Corporation. It is designed to protect the main analytical column from contamination and extend its lifetime. The pre-column features a bonded C18 stationary phase and is compatible with a wide range of solvents and mobile phases used in liquid chromatography applications.
Automatically generated - may contain errors
Lab products found in correlation
Acquity beh c18 column, by Waters Corporation (5 mentions)
Acquity uplc beh c18 vanguard pre column, by Waters Corporation (2 mentions)
Acquity ultra high performance liquid chromatography, by Waters Corporation (2 mentions)
Acquity tqd, by Waters Corporation (2 mentions)
Masslynx 4, by Waters Corporation (1 mentions)
Acquity uplc 1 class system, by Waters Corporation (1 mentions)
Xevo tq s micro mass spectrometer, by Waters Corporation (1 mentions)
9 protocols using acquity beh c18 pre column
1
UPLC-MS/MS Analysis of Target Analytes
2
PFAS Quantification by UPLC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The target analytes were analysed using an ACQUITY Ultrahigh Performance Liquid Chromatography (ACQUITY, TQD, Waters, Milford, MA, USA) coupled to a tandem quadrupole (TQD) mass spectrometer (UPLC-MS/MS) with negative electrospray ionisation. To separate the different target analytes, an ACQUITY UPLC BEH C18 VanGuard Precolumn (2.1 × 50 mm; 1.7 μm, Waters, USA) was used. The mobile phase solvents consisted of ACN and HPLC grade water, which were both dissolved in 0.1% HPLC grade formic acid. The solvent gradient started at 65% of water to 0% of water in 3.4 min and back to 65% water at 4.7 min. The flow rate was set to 450 μL/min and the injection volume was 6 μL. PFAS contamination that might originate from the LC-system was retained by insertion of an ACQUITY BEH C18 pre-column (2.1 × 30 mm; 1.7 μm, Waters, USA) between the solvent mixer and the injector. The target PFAS analytes were identified and quantified based on multiple reaction monitoring (MRM) of the diagnostic transitions that are displayed in Table S3.
3
UPLC-MS/MS Determination of Target Analytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The target analytes were analyzed using an ACQUITY Ultrahigh Performance Liquid Chromatography (ACQUITY, TQD, Waters, Milford, MA, USA) coupled to a TQD tandem quadrupole mass spectrometer (UPLC-MS/MS) with negative electrospray ionization. To separate the different target analytes, an ACQUITY UPLC BEH C18 VanGuard Pre-column (2.1 x 50 mm; 1.7 m, Waters, USA) was used. The mobile phase was composed of ACN, HPLC grade water and 0.1% HPLC grade formic acid. The solvent gradient started at 65% to 0% water in 3.4 min and back to 65% water at 4.7 min. The flow rate was set to 450 L/min and the injection volume was 10 L. PFAA contamination that might originate from the system was delayed by insertion of an ACQUITY BEH C18 pre-column (2.1 x 30 mm; 1.7 m, Waters, USA) between the solvent mixer and the injector. Each target analyte was identified and quantified based on multiple reaction monitoring (MRM) of the diagnostic transitions that are displayed in Table S2.
4
UHPLC-DAD-MS/MS Analysis of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All samples were run in duplicate. Measurement was conducted using UHPLC-DAD-MS/MS, with an Acquity UPLC I-Class system coupled to a XEVO-TQS micro mass spectrometer (Waters, Milford, MA, USA). The instrument consisted of a sample manager cooled at 10 °C, a binary pump, a column oven, and a diode array detector (DAD) measuring at 280 nm for confirmation of the peaks. The column oven temperature was set at 40 °C. Eluent B was water with 0.1% formic acid, eluent A was acetonitrile with 0.1% formic acid, and the flow was 0.4 mL/min on an Acquity BEH C18 RP column (50 mm × 2.1 mm, 1.7 µm particle size) combined with an Acquity BEH C18 precolumn (2.1 mm × 5 mm, 1.7 µm), both from Waters (Milford, MA, USA). The gradient started with 1% A for 3 min and rose linearly to 20% A within 1 min, then to 80% A within 1.3 min, then to 100% A in 0.3 min and holding for 1.5 min as a washing step; then back to 1% A within 0.2 min and equilibrating for 1 min. The injection volume was 5 µL.
The peaks were identified using MS/MS in negative mode. The source voltage was kept at 3.4kV, and the cone voltage was 77 V. The source temperature was set at 150 °C and the desolvation temperature at 400 °C with a desolvation gas flow of 800 L/h and a cone gas flow of 50 L/h. Data were acquired and processed using MassLynx 4.1 (Waters, Milford, MA, USA).
The peaks were identified using MS/MS in negative mode. The source voltage was kept at 3.4kV, and the cone voltage was 77 V. The source temperature was set at 150 °C and the desolvation temperature at 400 °C with a desolvation gas flow of 800 L/h and a cone gas flow of 50 L/h. Data were acquired and processed using MassLynx 4.1 (Waters, Milford, MA, USA).
5
PFAA Quantification by UPLC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After extraction, we analyzed the PFAAs using Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS, ACQUITY, TQD, Waters, Milford, MA, USA), with electrospray interface operating in negative ion mode (ES(-)-MS/MS). An ACQUITY BEH C18 column (2.1 × 50 mm; 1.7 μm, Waters, USA) separated the target analytes. An ACQUITY BEH C18 pre-column (2.1 × 30 mm; 1.7 μm, Waters, USA) was inserted between the solvent mixer and the injector to retain any PFAAs contamination originating from the system. The mobile phase solvents were A) 0.1 % formic acid in water and B) 0.1 % formic acid in ACN. The flow rate was set at 450 μl min -1 with an injection volume of 10 μl. The gradient started at 65 % A/35% B, decreased in 3.4 min to 0 % A/90% B and returned to 65% A/35% B at 4.7 min. The targeted PFAAs were identified and quantified by multiple reaction monitoring (MRM) using two diagnostic transitions per target analyte. The diagnostic transitions used are listed in Table S2.
6
PFAA Quantification via UPLC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A N U S C R I P T 9 with a solvent gradient starting at 65% A to 0% A in 3.4 min to 65% A at 4.7 min and a flow rate of 450 L/min and an injection volume of 10 L. An ACQUITY BEH C18 pre-column (2.1 x 30 mm; 1.7 m, Waters, USA) was inserted, between the solvent mixer and injector, to retain any PFAAs contamination originating from the system. PFAAs were identified and quantified based on multiple reaction monitoring (MRM) of the following diagnostic transitions: 213 169 (PFBA),217 172 ( 13 C4PFBA),263 219 (PFPeA)
7
Comprehensive PFAA Analysis by UPLC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We analyzed PFAAs by UPLC coupled tandem ES(-) mass spectrometry (ACQUITY, TQD, Waters, Milford, MA, USA) using an ACQUITY BEH C18 column (2.1 X 50 mm; 1.7µm, Waters, USA), mobile phase: 0.1 % formic acid in water(A), 0.1 % formic acid in acetonitrile(B), solvent gradient: from 65% A to 0 % A in 3.4 min and back to 65%A at 4.7 min, flow rate: 450 μL/min, injection volume: 10 µl. To retain any PFAA contamination originating from the system, we inserted an ACQUITY BEH C18 pre-column (2.1 × 30 mm; 1.7 μm, Waters, USA) between the solvent mixer and the injector. Identification and quantification was based on multiple reaction monitoring (MRM) of the following diagnostic transitions: 213 169 (PFBA), 313 296 (PFHxA), 315 270 ( 13 C2-PFHxA), 413 369 (PFOA), 421 376 ( 13 C8-PFOA), 463 419 (PFNA), 472 427 ( 13 C9-PFNA), 513 469 (PFDA), 519 474 ( 13 C6-PFDA), 613 569 (PFDoA), 613 319 (PFDoA), 615 169 ( 13 C7PFDoA), 615 570 ( 13 C7PFDoA), 570 525 ( 13 C7PFUdA), 663 619 (PFTrA), 713 669 (PFTeA), 713 369 (PFTeA), 299 99 (PFBS), 399 99 (PFHxS), 403 103 ( 18 O2-PFHxS), 599 80 (PFDS), 499 80 (PFOS), 499 99 (PFOS) and 507 80 ( 13 C8-PFOS).
8
Targeted Quantification of PFAAs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We used ultra-performance liquid chromatography coupled tandem ES(-) mass spectrometry (ACQUITY, TQD, Waters, Milford, MA, USA) to analyze the target analytes. Individual PFAAs were separated using an ACQUITY BEH C18 column (2.1 x 50 mm; 1.7 µm, Waters, USA) and an ACQUITY BEH C18 pre-column (2.1 x 30 mm; 1.7 µm, Waters, USA) was inserted between the solvent mixer and injector to retain PFAA contamination from the system. As mobile phase solvents we used A) 0.1% formic acid in water and B) 0.1% formic acid in ACN. The injection volume was 10 µL at a flow rate of 450 µL/min. The gradient started at 65% A, decreased to 0% A in 3.4 min and returned to 65% A at 4.7 min. To identify and quantify the target PFAAs, multiple reaction monitoring (MRM) of two diagnostic transitions per target analyte (Table 1) was used.
9
PFAA Analysis via UPLC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To analyze the PFAAs, we used ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS, ACQUITY, TQD, Waters, Milford, Ma, USA). Target analytes were separated using an ACQUITY BEH C18 column (2.1 x 50 mm; 1.7 m, Waters, USA) and an ACQUITY BEH C18 pre-column (2.1 x 30 mm; 1.7 m, Waters, USA) was inserted between the solvent mixer and the injector to retain any PFAAs contamination originating from the system. The mobile phase solvents were A) 0.1% formic acid in water and B) 0.1% formic acid in ACN. The flow rate was set at 450 L/min with an injection volume of 10 L. The gradient started at 65% A, decreased in 3.4 min to 0% A and returned to 65% A at 4.7 min. Multiple reaction monitoring (MRM) of two diagnostic transitions per target analyte was used to identify and quantify the PFAAs. The diagnostic transitions are displayed in Table 1.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!