Total RNA was extracted from the plasma of 150 healthy controls, 165 NSTEMI patients, and 135 STEMI patients using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and according to the manufacturer's instructions. A Quantitative real-time polymerase chain reaction (qRT-PCR) of hsa-miR-6735-3p, hsa-miR-3677-5p, lncRNA MALAT1 and hsa-miR-1972, hsa-miR-194-5p, and hsa-miR-4717-5p was performed on a Bio-Rad CFX96 thermal cycler using SYBR Green Q-PCR Master Mix (TaKaRa, Dalian, China). Based on the primer information in Table 1, U6 was used as an internal control. The expression levels of lncRNA MALAT1 and hsa-miR-1972, hsa-miR-194-5p, hsa-miR-4717-5p, hsa-miR-6735-3p, and hsa-miR-3677-5p were normalized to U6. Each sample was tested in triplicate.
Cfx96 thermal cycler
Manufactured by Takara Bio
Sourced in China
The CFX96 thermal cycler is a real-time PCR detection system designed for DNA amplification and quantification. It features a 96-well sample block and can perform various qPCR applications, including gene expression analysis, genotyping, and pathogen detection. The CFX96 provides precise temperature control and supports a wide range of sample volumes and reaction types.
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Lab products found in correlation
2 protocols using cfx96 thermal cycler
1
Plasma miRNA and lncRNA Profiling in ACS
2
Quantifying PIK3CA Expression in PBMCs
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Total RNA was extracted from peripheral blood mononuclear cells (PBMCs) of 60 patients using Trizol reagent (Invitrogen, USA) according to the manufacturer's instructions. Quantitative real-time polymerase chain reaction (qRT-PCR) for PIK3CA was performed on Bio-Rad CFX96 Thermal Cycler using SYBR Green Q-PCR Master Mix (Takara, Dalian, China). The primers used in qRT-PCR were as follows: 5′-GGAGCCTGGAAGAGCCC-3′ (F); 5′-CGTGGAGGCATTGTTCTGAT-3′ (R). The qRT-PCR was performed as described previously [9 (link)].
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