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2 protocols using anti asc sc 22514

1

Antibody Procurement for Protein Analysis

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Antibodies against pro–IL-1β (12242) and cleaved IL-1β (83186) were purchased from Cell Signaling Technology. Antibodies against tubulin (AG-27B-0005-C100), caspase-1 (AG-20B-0048-C100), and NLRP3 (AG-20B-0014) were purchased from Adipogen. Antibodies against gasdermin-D (ab210070), HSP90β (ab53497), and HSP90α (ab79849) were purchased from Abcam. Anti-SGT1 (612104) was purchased from BD Biosciences. Anti–vesicular stomatitis virus glycoprotein (VSVg) (V4888) was purchased from Sigma-Aldrich. Anti-ASC (sc-22514) was purchased from Santa Cruz Biotechnology. Anti-mouse–horseradish peroxidase (HRP) (115-035-146), anti-rabbit–HRP (111-035-144), and anti-human–HRP (709-035-149) were purchased from the Jackson Laboratory. Anti-rabbit–Alexa Fluor 647 was purchased from Thermo Fisher Scientific.
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2

Antibody Staining Protocol for Immune Cell Analysis

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Antibodies to GSDMD (ab219800 and ab209845) and MUC2 (AB76774) were from Abcam. Anti–α-tubulin (T9026) and anti–β-actin (A1978) were from Sigma-Aldrich. Anti–caspase-11 (NB120-10454) was supplied from Novus Biologicals. Anti-ASC (sc-22514) was from Santa Cruz Biotechnology. Anti-phosphorylated STING (72971s), anti-STING (13647s), anti-phosphorylated IRF3 (4947s), anti-IRF3 (4302s), anti-TBK1 (3013s), and anti-phosphorylated TBK1 (5483s) were from Cell Signaling Technology. DSS (DB001-38) was from TdB Consultancy. PAS staining kit was from Servicebio (G1008). Alcian blue staining kit was from Solarbio (G1560). Anti–CD45–fluorescein isothiocyanate (FITC) (30-F11,11-0451-82), anti–CD8a–phycoerythrin (PE) (53-6.7,12-0081-83), anti–CD45-AF700 (30-F11,85-11-0112-81), anti–CD11b-FITC (M1/70,85-12-0114-81), anti-F4/80 (BM8,17-4801-82), anti–Ly6c-PE-Cy7 (HK1.4,25-5932-82), anti–Ly6g–eFlour 450 (1A8-LY6G,48-9668-82), and FVD (fixable viability dyes)–eFlour 506 (65-0866) were from eBioscience. Anti–CD4-APC (allophycocyanin)–Cy7 (GK1.5,100414) was from BioLegend. Anti–CD11b-PE (M1/70, 557397) was from BD Biosciences.
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