HDAC1–9 enzyme activity was measured using HDAC fluorogenic assay kits (BPS Bioscience). Increasing dilutions of HDAC inhibitors were incubated with HDAC substrate, enzyme and buffer at 37°C for 30 min. Fluorescence was measured using a microplate reader.
Hdac fluorogenic assay kits
Manufactured by BPS Biosciences
The HDAC fluorogenic assay kits from BPS Biosciences are designed to measure the activity of histone deacetylase (HDAC) enzymes. The kits utilize a fluorogenic substrate that emits a fluorescent signal upon HDAC-mediated deacetylation. This allows for the quantitative determination of HDAC enzyme activity in a simple, homogeneous assay format.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Hdac6, by Cell Signaling Technology (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase, by Cell Signaling Technology (1 mentions)
Hdac11, by BPS Biosciences (1 mentions)
Hdac2, by BPS Biosciences (1 mentions)
Hdac1, by BPS Biosciences (1 mentions)
Hdac6, by BPS Biosciences (1 mentions)
Amersham ecl select western blotting detection reagent, by GE Healthcare (1 mentions)
Dulbecco s modified eagle s medium dmem with l glutamine, by GenDEPOT (1 mentions)
Goat anti rabbit igg horseradish peroxidase conjugate, by Santa Cruz Biotechnology (1 mentions)
Hdac4, by BPS Biosciences (1 mentions)
Hdac3, by BPS Biosciences (1 mentions)
Celltiter 96 aqueous one solution cell proliferation assay kit, by Promega (1 mentions)
2 protocols using hdac fluorogenic assay kits
1
HDAC Enzyme Activity Assay
2
Histone Deacetylase Assays and Immunoblotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dulbecco's modified Eagle's medium (DMEM) with L-glutamine was purchased from GenDEPOT (Barker, TX), fetal bovine serum was purchased from HyClone, and penicillin and streptomycin were purchased from Gibco BRL (Gaithersburg, MD). Antibodies for α-tubulin, Ac-α-tubulin (Lys40), Histone H3, Ac-Histone H3 (Lys9), HDAC1, HDAC6, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Cell Signaling Technology (Boston, MA). Goat anti-rabbit IgG horseradish peroxidase conjugate was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Cell Titer 96 Aqueous One Solution cell proliferation assay kit was purchased from Promega (Madison, WI). Amersham ECL Select western blotting Detection Reagent was purchased from GE Healthcare. HDAC fluorogenic assay kits (HDAC1, #50061; HDAC2, #50062; HDAC3, #50073; HDAC4, #50064; HDAC6, #50076; HDAC11, #50687) were bought from BPS Bioscience (San Diego, CA).
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