For plasmid transfection, VICs were seeded at 5,000 cells/well into 96-well (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted September 6, 2021. ; https://doi.org/10.1101/2021.09.06.459126 doi: bioRxiv preprint culture plates, transfected with either an empty vector (Solarbio, China) or a Slc7a11 cDNA ORF Clone (NM_014331.3) (SinoBiological, China) using Lipofectamine 3000 Transfection Reagent (Invitrogen, USA) and cultured for 24 h in minimal medium comprising DMEM and 5% FBS without antibiotics. Then, the VICs were harvested for transient transfection analysis, and successful overexpression was confirmed by RT-PCR.
The copyright holder for this preprint this version posted September 6, 2021. ; https://doi.org/10.1101/2021.09.06.459126 doi: bioRxiv preprint culture plates, transfected with either an empty vector (Solarbio, China) or a Slc7a11 cDNA ORF Clone (NM_014331.3) (SinoBiological, China) using Lipofectamine 3000 Transfection Reagent (Invitrogen, USA) and cultured for 24 h in minimal medium comprising DMEM and 5% FBS without antibiotics. Then, the VICs were harvested for transient transfection analysis, and successful overexpression was confirmed by RT-PCR.