The crude extracts of K. alvarezii and C. serrulata were loaded into a silica gel (Hi-Media) packed column (20 cm length and 2 cm diameter) and eluted using n-hexane: ethyl acetate (50:50 v/v) (Sigma-Aldrich). The fractions were analyzed via a gas chromatograph GC-2010 interfaced with a quadrupole mass spectrometer QP-2010 (Shimadzu, Japan) analyzer, which used an Rtx-PCB capillary column (60 m × 0.25 mm i.d., 0.25 mm film thickness, Resteck, Bellefonte, PA, USA). Helium with a purity of 99.99% was used as the carrier gas at a flow rate of 1 mL/min. Next, 1 mL of extract was injected in spilt mode using an autosampler (Shimadzu). The injector port, interface and ion source temperature were set at 250, 270 and 230 °C, respectively. The mass spectrometer was operated in electron ionization (EI) mode at 70 eV and at an emission current of 60 mA. Full scan data were obtained in a mass range of 50–500 m/z. Interpretation of mass spectrum data was performed using the National Institute Standard and Technology (NIST) database.
N hexane ethyl acetate
Manufactured by Merck Group
Sourced in United States
N-hexane: ethyl acetate is a solvent mixture commonly used in laboratory settings. It serves as a mobile phase in various chromatographic techniques, such as thin-layer chromatography (TLC) and column chromatography, to separate and purify organic compounds. The mixture's composition and ratio can be adjusted based on the specific application and the properties of the compounds being analyzed or purified.
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2 protocols using n hexane ethyl acetate
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Phytochemical Characterization of Seaweed Extracts
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HPLC-based Acrylamide Quantification in Food Samples
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HPLC-grade organic solvents were exclusively used in this study. N-hexane ethyl acetate, methanol were supplied by Sigma-Aldrich (Belefonte, PA, USA). Anhydrous sodium sulphate, hydrogen bromide, potassium bromide, bromine water, acetic acid, zinc acetate, hexacyanoferrate, sodium thiosulphate, borate buffer pH 8 of p. a. grade were supplied by Chempur (Piekary Slaskie, Poland). Florisil (60-100 mesh), triethylamine were supplied by Sigma-Aldrich. C18 (500 mg, 6 ml), Strata X-C (150 mg, 6 ml), Bond-Elute Plexa PCX (200 mg, 6 ml) solid phase extraction cartridges (SPE) were supplied by Agilent Technologies (Santa Clara, CA, USA) and Phenomenex (Torrance, CA, USA). Water used in the laboratory procedures was purified using Hydrolab water purification system (Wiślina, Poland). Certified reference material of acrylamide in rusk (ERMBD274, BAM, Berlin, Germany) was supplied by Sigma-Aldrich. Native and deutered (D 3 ) AA standards were supplied by Sigma-Aldrich. Standards were prepared in ultrapure water. AA certified reference material ERMBD274 was supplied by Sigma-Aldrich. Carrez solution #1 was prepared by dissolving 106 g of potassium hexacyanoferrate trihydrate in 1000 ml of water. Carrez solution #2 was prepared by dissolving 219 g of zinc acetate dihydrate in 1000 ml of water.
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