Equisplash lipidomix quantitative mass spec internal standard

Manufactured by Avanti Polar Lipids

EquiSPLASH® LIPIDOMIX® is a quantitative mass spectrometry internal standard product offered by Avanti Polar Lipids. It is designed to be used as a reference material for lipidomics analysis.

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Lab products found in correlation

Debrisoquine, by Merck Group (1 mentions) Hexosylceramides, by AB Sciex (1 mentions) Lactosylceramides, by AB Sciex (1 mentions) Fisher optima grade, by Thermo Fisher Scientific (1 mentions) High purity formic acid, by Thermo Fisher Scientific (1 mentions) 4 nitrobenzoic acid, by Merck Group (1 mentions) 18 1 chol d7 ester, by Avanti Polar Lipids (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) 2 propanol, by Thermo Fisher Scientific (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) α mycolic acid c 80, by Avanti Polar Lipids (1 mentions)

Close spelling variants of this product

EquiSplash Lipidomix Internal standards EquiSPLASH

2 protocols using equisplash lipidomix quantitative mass spec internal standard

Comprehensive Lipidomics Analysis Protocol

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All LC-MS grade solvents including acetonitrile and water were purchased from Fisher Optima grade, Fisher Scientific. High purity formic acid (99%) was purchased from Thermo-Scientific. Debrisoquine and 4-nitrobenzoic acid were purchased from Sigma-Aldrich. EquiSPLASH^® LIPIDOMIX^® quantitative mass spec internal standard and 15:0-18:1-d7-PA, C15 Ceramide-d7 (d18:1-d7/15:0) and 18:1 Chol (D7) ester were purchased from Avanti polar lipids. Internal standard for free fatty acid (FFA), dihydroceramides (DCER), hexosylceramides (HCER), lactosylceramides (LCER) were purchased from Sciex as Lipidyzer platform kit. The LC-MS data acquisition details are provided in Supplementary data.

Bansal S., Bansal S., Fish B.L., Li Y., Xu X., Fernandez J.A., Griffin J.H., Himburg H.A., Boerma M., Medhora M, & Cheema A.K. (2023). Analysis of the Urinary Metabolic Profiles in Irradiated Rats treated with Activated Protein C (APC), a Potential Mitigator of Radiation Toxicity. International journal of radiation biology, 99(7), 1109-1118.

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Lipidomics Internal Standard Quantification

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EquiSPLASH™ LIPIDOMIX® Quantitative Mass Spec Internal Standard, α-mycolic acid (C80) and methoxy cis α-mycolic acid were purchased from Avanti Polar Lipids (Birmingham, AL). Optima LC-MS grade acetonitrile plus 0.1% formic acid, water plus 0.1% formic acid, methanol and 2-propanol were purchased from Fisher Chemical (Waltham, MA). LC grade chloroform was purchased from Sigma (St. Louis, MO). All other chemicals were purchased from Sigma (St. Louis, MO) and were of analytical grade quality. A 10 mM stock solution of ammonium formate at pH 6.2 was used as a standard buffer.

Sakallioglu I.T., Maroli A.S., Liete A.D, & Powers R. (2021). A Reversed Phase Ultra-High-Performance Liquid Chromatography-Data Independent Mass Spectrometry Method for the Rapid Identification of Mycobacterial Lipids. Journal of chromatography. A, 1662, 462739.

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