Inhibitory human neurons were fixed for 15 minutes in 4% paraformaldehyde in phosphate buffered saline (PBS) and permeabilized using 0.1% Triton X-100 in PBS for 10 minutes at room temperature. Cells were then incubated in blocking buffer (4% bovine serum albumin (BSA) with 1% normal goat serum in PBS) for 1 hour at room temperature and then incubated with primary antibodies diluted in blocking buffer for 1 hour at room temperature, washed with PBS three times, and subsequently incubated in secondary antibodies for 1 hour at room temperature. Confocal imaging analysis was performed using a Zeiss LSM700. Primary Antibodies used include: mouse anti Oct4 (Millipore Sigma MAB4401, 1:2000), mouse anti Tra-1-60 (Millipore Sigma MAB4360, 1:1000), mouse anti MAP2 (Sigma-Aldrich M1406, 1:500), rabbit anti MAP2 (Sigma-Aldrich M3696, 1:500), chicken anti MAP2 (Millipore AB5543, 1:1000), rabbit anti Synapsin (e028, 1:3000), rabbit anti VGAT (Millipore Sigma AB5062P, 1:2000), mouse anti Gad-67 (Abcam ab26116, 1:500), mouse anti β3 Tubulin (BioLegend 801201, 1:2000), mouse anti Calbindin (Abcam ab82812, 1:500), guinea pig anti Parvalbumin (Swant GP72, 1:2500), rat anti Somatostatin (Millipore MAB354, 1:50).
Sigma mab4401
Manufactured by Merck Group
Sigma MAB4401 is a monoclonal antibody produced in mouse and purified from mouse ascites fluid. It is designed for use in immunohistochemical and immunofluorescent detection applications.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 700, by Zeiss (4 mentions)
Ab82812, by Abcam (2 mentions)
Ab26116, by BioLegend (2 mentions)
Mab354, by Merck Group (2 mentions)
Ab5543, by Merck Group (2 mentions)
Sigma mab4360, by Merck Group (2 mentions)
M3696, by Merck Group (2 mentions)
Sigma ab5062p, by Abcam (2 mentions)
M1406, by Merck Group (2 mentions)
2 protocols using sigma mab4401
1
Immunofluorescent Analysis of Inhibitory Neurons
2
Immunofluorescent Analysis of Inhibitory Neurons
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Inhibitory human neurons were fixed for 15 minutes in 4% paraformaldehyde in phosphate buffered saline (PBS) and permeabilized using 0.1% Triton X-100 in PBS for 10 minutes at room temperature. Cells were then incubated in blocking buffer (4% bovine serum albumin (BSA) with 1% normal goat serum in PBS) for 1 hour at room temperature and then incubated with primary antibodies diluted in blocking buffer for 1 hour at room temperature, washed with PBS three times, and subsequently incubated in secondary antibodies for 1 hour at room temperature. Confocal imaging analysis was performed using a Zeiss LSM700. Primary Antibodies used include: mouse anti Oct4 (Millipore Sigma MAB4401, 1:2000), mouse anti Tra-1-60 (Millipore Sigma MAB4360, 1:1000), mouse anti MAP2 (Sigma-Aldrich M1406, 1:500), rabbit anti MAP2 (Sigma-Aldrich M3696, 1:500), chicken anti MAP2 (Millipore AB5543, 1:1000), rabbit anti Synapsin (e028, 1:3000), rabbit anti VGAT (Millipore Sigma AB5062P, 1:2000), mouse anti Gad-67 (Abcam ab26116, 1:500), mouse anti β3 Tubulin (BioLegend 801201, 1:2000), mouse anti Calbindin (Abcam ab82812, 1:500), guinea pig anti Parvalbumin (Swant GP72, 1:2500), rat anti Somatostatin (Millipore MAB354, 1:50).
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