MIG6 knockdown ECs were seeded onto gelatin-coated 8W10E ECIS array (Applied Biophysics, MA, USA) and allowed to reach confluence overnight. Following low serum starvation (1% FBS) for 3 h, permeability was measured after VEGFA treatment (50 ng/ml) using multiple frequency/time (MFT) setting. MIG6 expression was confirmed by western blot analysis.
8w10e ecis array
Manufactured by Applied Biophysics
Sourced in United States
The 8W10E ECIS arrays are a type of lab equipment used for electrical cell-substrate impedance sensing (ECIS) measurements. These arrays provide a platform for monitoring cellular behavior and adhesion through the real-time measurement of electrical impedance. The core function of the 8W10E ECIS arrays is to enable researchers to collect and analyze data on various cellular properties, such as cell attachment, spreading, and motility, without the need for labeling or invasive techniques.
Automatically generated - may contain errors
7 protocols using 8w10e ecis array
1
Measuring EC Permeability Dynamics
2
Measuring Endothelial Barrier Function
3
Resolvin D1 Modulates Barrier Function in A549 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A549 cells were seeded on 8W10E+ ECIS arrays (Applied Biophysics, Troy, NY) at a density of 0.5 x 106 cells/mL. Cells were grown to a confluence as measured by a reading of 10 μF on the ECIS System (Applied Biophysics, Troy, NY). After achieving confluence, A549 cells were subjected to serum starvation and were treated with IL-1β (10 ng/mL) in the presence or absence of AT-RvD1 (100 nM) for 6 hours. Cells receiving resolvins were pretreated with AT-RvD1 for 30 minutes prior to addition of IL-1β or vehicle. Resistance measurements were taken for a period of 48 hours. Three samples were used and 4 measurements were taken at each time point to mitigate inter- and intra- sample variability. Culture medium served as the electrode and barrier function was analyzed as a function of impedance of a cell-covered electrode. A 1-V, 4,000-Hz alternating current signal was supplied through a 1-MΩ resistor to approximate a constant-current source. ECMS 1.0 software (CET, Coralville, IA) was used to measure the in-phase voltage resistance and capacitance. Epithelial barrier function of A549 cells was expressed as TER, which was normalized to a time-zero baseline. Epithelial monolayers that did not achieve a baseline TER of 5,000 Ω or higher were excluded from this study.
4
Evaluating 4T1 Cell Wound Healing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The ECIS assay was used to measure the healing ability of the 4T1 cells (21 (link)). A 400 µl suspension of 4T1 with 8×104 cells were seeded in 8W10E+ ECIS arrays (Applied Biophysics, Inc., Troy, NY, USA) in each well. Following inoculation of the cells, the procedure of attachment and spreading was exhibited by impedance measurements. Lethal electroporation (current, 6,500 µA; frequency, 100,000 Hz; time, 60 sec) was performed when the cells were fully confluent (~16 h). The dead cells were washed away and fresh medium containing ELE (25 µg/ml) and LMWH (200 IU/ml) was added. The wound healing was assessed by continuous impedance measurements for 20 h. The experiment was performed in a humidified 5% CO2 incubator at 37°C and was repeated three times.
5
Quantitative Wound Healing Assay Using ECIS
6
Measuring HLMVEC Integrity with ECIS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TER assays were performed to measure HLMVEC integrity using ECIS instrument Z-Theta model (Applied BioPhysics, Troy, NY) as described previously (Garcia et al., 2000 (link)). The cells were grown in 8-well 8W10E ECIS arrays (Applied BioPhysics) to 100%-confluency in complete EGM-2 medium. The cells were pre-treated with HDAC inhibitors or vehicle as a control for 30 min, then the cells were challenged with LPS, and TER response changes were real-time recorded. Initial resistance in all array wells was normalized to 1.
7
Real-time Wound Healing Assay Using ECIS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Real-time quantitative wound-healing assays were performed using the Electric Cell-substrate Impedance Sensing (ECIS; model 1600R; Applied BioPhysics, Troy, NY) and the 8W10E ECIS arrays. MDA-MB-435S cells were treated with A769662 or Compound C for 48 h followed by treatment with Mitomycin C for 2 h to arrest proliferation, trypsinized and a total of 2×105 live cells seeded in three wells of the 8W10E ECIS array. One well with only medium (no cells) served as a negative control to provide the baseline changes in impedance. Cells were allowed to attach for 24 h, after which a wound was made using an elevated AC voltage pulse with a frequency of 40 kHz, and duration of 30 s. Dead cells were washed away by replacing the medium. Migratory response over the next 24 h was measured in real time by recording the recovery of electrical impedance.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!