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2 protocols using ampicillin sodium

1

Plasmid Extraction and DNA Electrophoresis

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Plasmids were extracted using the FavorPrep™ Plasmid DNA Extraction Mini Kit (FAVORGEN Biotech Corp., Taiwan). DNA-Staining Bandi Load (6 × ) was purchased from Smartgene (SJ Bioscience, Daejeon, Korea). The ExcelBand™ 1 KB DNA ladder (DM3100; SMOBIO Technology) was used as the DNA size marker. Moreover, 50 × Tris-acetate-EDTA (TAE) buffer and 20 × phosphate-buffered saline (PBS) were purchased from Dongin Biotech (Seoul, Korea). Ampicillin sodium and kanamycin sulfate monohydrate were purchased from Duchefa (Duchefa Biochemie, Netherlands). Luria–Bertani agar (LB agar) and LB broth were purchased from Difco (Miller, BD, Franklin Lakes, NJ, USA).
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2

Rapid Fluorescent Immunoassay for Enrofloxacin Detection

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Enrofloxacin, ciprofloxacin, ofloxacin, norfloxacin, tetracycline, creatine monohydrate, bovine serum albumin (BSA), polyvinyl alcohol (PVA), Tween 20, and Gold (III) chloride trihydrate (HAuCl4.3H2O, ≥99.9) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Ampicillin sodium and chloramphenicol were purchased from Duchefa Biochemie (Haarlem, Netherlands). Trisodium citrate dihydrate was purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Anti-Enrofloxacin monoclonal antibody (ENR-Ab) produced in mice and coating antigen BSA-ENR were purchased from Creative Diagnostics, (New York, USA). The backing card, absorbent pad, sample pad, nitrocellulose membrane, and conjugation pad used to fabricate LFIA strips, were purchased from Bore Da Biotech Co., Ltd. (Seongnam, Korea). Milk samples were purchased from a local market in Incheon, South Korea. All other reagents and solvents used were of analytical grade or higher.
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