Lamp1 antibody h4a3

Cambinol, Actinomycin D, TSA, Rapamycin, and Nicotinamide were purchased from Sigma (St. Louis, MO). AK1 and AGK2 were purchased from ChemBridge (San Diego, Ca). EX527 was supplied by Selleck Chemicals (Houston, TX). UO126 was purchased from Calbiochem (Billerica, MA) and used at 10 μM. LAMP-1 antibody (h4A3) was obtained from the Developmental Studies Hybridoma Bank at the University of Iowa and used at a 1:200 dilution. GM130 and EEA1 antibodies (BD Biosciences, San Jose, CA) were used at 1:50. Mitotracker Red (Molecular Probes, Carlsbad, CA) was used according to the manufacturer's protocol. The SIRT1 (B-10) and Ac-tubulin antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX) and was used at 1:1000. pMEK1/2 S271/221, p70 S6 Kinase Thr389, pErk1/2 T202/Y204, Ac-p53 Lys382, LC3I/II, and total Erk1/2 antibodies (Cell Signaling Technology, Beverly, MA) were used at 1:1000 for western blot. Anti-tubulin antibody (NeoMarkers, Fremont, CA) was used at 1:100 for immunofluorescence (IF) and 1:20,000 for western blot analysis. Secondary antibodies include Dylight 594 donkey anti-mouse 1:100 (Jackson IR, West Grove, PA) for immunoflorescence, and HRP-conjugated anti-mouse and anti-rabbit for western blot (GE Healthcare, Pittsburgh, PA) used at 1:5000. Phalloidin 488 (Invitrogen, Carlsbad, CA) was used at 1:200.