HbA
1c was analysed locally at each centre, using DCCT aligned methods (HbA
1c Variant analyser [Bio-Rad Laboratories, Hercules, CA, USA], Adams Arkray [Kyoto, Japan],
Vantage analyser [Siemens Diagnostics, Camberley, UK] or
DCA 2000, [Siemens Diagnostics, Tarrytown, NY, USA]). Lipid profile measurements (cholesterol, HDL-cholesterol, LDL-cholesterol, triacylglycerol) were measured using routine laboratory methods.
The standard deviation score (SDS) was calculated for height, weight and BMI according to the least-mean squares method [17 (
link)]. Blood pressure was measured using an Omran M6 blood pressure monitor (Hoofddorp, the Netherlands) and/or Dinamap monitor (Tampa, FL, USA) using an appropriate sized cuff. Age- and sex-related percentiles and SDS for systolic blood pressure (SBP) and diastolic blood pressure (DBP) were calculated according to published standards [18 (
link)].
This study was approved by the Human Research Ethics Committees of each participating centre. Informed consent was obtained from participants and their families.
Benitez-Aguirre P.Z., Wong T.Y., Craig M.E., Davis E.A., Cotterill A., Couper J.J., Cameron F.J., Mahmud F.H., Jones T.W., Hodgson L.A., Dalton R.N., Dunger D.B, & Donaghue K.C. (2018). The Adolescent Cardio-Renal Intervention Trial (AdDIT): retinal vascular geometry and renal function in adolescents with type 1 diabetes. Diabetologia, 61(4), 968-976.