The Ovsynch procedure was used to synchronize lactating nonpregnant primiparous (n = 31) and multiparous (n = 51) Holstein cows at various DIMs. Estrus was synchronized using ovsynch protocol [4 (link)]. The experimental cows received 10 μg of a GnRH analogue (Buserelin acetate: Receptal®;MSD animal health, Egypt) intramuscularly followed 7 days later with an intramuscular injection of (500 μg cloprostenol sodium; Estrumate intramuscularly; Essex, Munich, Germany), and given 10 μg, injection of the GnRH analogue i.m. (2.5ml/animal) 48h after the PGF2aα treatment and the cows were bred 14-16 h then after. The ovaries were examined ultasonographically 12, 24, and 36 h later to confirm that ovulation has occurred. Day 1 of the estrous cycle was defined as the day when the dominant follicle was no longer detectable due to it having ovulated and the pregnancy check was done by ultrasound imaging at day 33 post inseminations.
Receptal
Manufactured by MSD animal health
Sourced in New Zealand
Receptal is a prescription veterinary product used for the synchronization of estrus and ovulation in cattle and sheep. It contains gonadorelin diacetate tetrahydrate as the active ingredient.
Automatically generated - may contain errors
5 protocols using receptal
1
Ovsynch Synchronization in Dairy Cows
2
Ovulation Synchronization in Repeat-Breeder Cows
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The short-term 5-day P4-PGF-GnRH-based programme of the present study was adapted from a previous study [18 (link)]. At the beginning of the hormonal protocol for ovulation synchronisation (day 0), a total of 60 selected repeat-breeder dairy cows with ovarian inactivity were implanted with an exogenous P4-releasing device with 1.38 g of P4 (controlled internal drug release (CIDR), Eazi-Breed; Zoetis Inc., Mahuhu Crescent, Auckland, New Zealand), concurrent with an injection of P4 (50 mg; Steraloids, Inc., Newport, RI, USA), and CIDR inserts were removed from the vagina on day 5. On day 5, all dairy cows received a 250 µg dose (cloprostenol) of PGF (Estrumate, MSD Animal Health, Upper Hutt, Wellington, New Zealand). On day 8, a 10 µg dose (buserelin) of GnRH (Receptal, MSD Animal Health, Upper Hutt, Wellington, New Zealand) was administered concurrently with the FTAI in all dairy cows. The FTAI was performed on day 8 using a single dose of frozen-thawed semen. The completion of hormonal synchronisation period was designated as day 8.
3
Synchronization protocol for fixed-time AI in cows
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All cows were subjected to intramuscular treatment with gonadotropin releasing hormone GnRH; 20µg buserelin acetate (5 ml Receptal; Zoetis, New Zealand) at day 0 then cows were injected intramuscularly with 500µg Cloprostenol sodium (PGF2α) (2 ml Estrumate® MSD animal Health Egypt) ) at day 7 then at day 9 repeat the GnRH (5 ml Receptal) then after timed AI at the 10 th day (16-20 hours after the 2 nd GnRH injection.
synchronization program for fixed time artificial insemination.
synchronization program for fixed time artificial insemination.
4
Estrus Synchronization in Buffaloes
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Animals were randomly assigned into two groups based on the protocol of estrus synchronization [11 (link)]. The first group (CIDR-PGF2α group, n = 18)
received CIDR device impregnated with 1.38 mg progesterone (EAZIBREED, inter Age, Hamilton, New Zealand) inserted intra-vaginally for 7 days and an intramuscular injection of a synthetic
prostaglandin F2α analogue (PGF2α; 500 µg cloprostenol sodium, Estrumate, Intervet, Merck Animal Health, USA) one day before the CIDR withdrawal. The second group (Ovsynch-CIDR group, n =
18) received treatment according to the Ovsynch protocol, accompanied by an intra-vaginal insertion of CIDR for 7 days. Briefly, two doses of 20 μg of gonadotropin-releasing hormone analog
(GnRH; 5.0 ml Receptal, MSD Animal Health, Intervet International GmBH, Germany) were given intramuscularly seven days before and two days after a single dose of PGF2α. After the end of the
treatment, all buffaloes were checked for behavioral signs of estrus by a vasectomized bull twice a day (at 12 h interval) to stimulate estrus behavior. All buffaloes were subjected to timed
AI 12 h after synchronized estrus onset using frozen-thawed semen from a known fertile bull.
received CIDR device impregnated with 1.38 mg progesterone (EAZIBREED, inter Age, Hamilton, New Zealand) inserted intra-vaginally for 7 days and an intramuscular injection of a synthetic
prostaglandin F2α analogue (PGF2α; 500 µg cloprostenol sodium, Estrumate, Intervet, Merck Animal Health, USA) one day before the CIDR withdrawal. The second group (Ovsynch-CIDR group, n =
18) received treatment according to the Ovsynch protocol, accompanied by an intra-vaginal insertion of CIDR for 7 days. Briefly, two doses of 20 μg of gonadotropin-releasing hormone analog
(GnRH; 5.0 ml Receptal, MSD Animal Health, Intervet International GmBH, Germany) were given intramuscularly seven days before and two days after a single dose of PGF2α. After the end of the
treatment, all buffaloes were checked for behavioral signs of estrus by a vasectomized bull twice a day (at 12 h interval) to stimulate estrus behavior. All buffaloes were subjected to timed
AI 12 h after synchronized estrus onset using frozen-thawed semen from a known fertile bull.
5
Synchronized Estrus Cycle in Holstein-Friesian Heifers
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Eight Holstein-Friesian heifers (15–17 months old) were oestrus-synchronised using Eazi-Breed™ CIDR® Cattle Insert (1.38 g progesterone over eight days; Zoetis, USA), Receptal® (0.02 mg buserelin on the day of CIDR insertion; MSD Animal Health, UK) and Estrumate® (0.5 mg cloprostenol seven days after CIDR insertion; MSD Animal Health). Blood was collected on Days 0, 8 and 16 from all animals (Day 0 being the first day of observed oestrus). The stage of the oestrous cycle was confirmed by plasma progesterone profiles determined using a Coat-a-Count radio-immuno-assay (Siemens Healthcare, Germany). All animal procedures were carried out under the UK Home Office Animals (Scientific Procedures) Act 1986, license 60/4604, and with approval by the Ethical Review Committee, University of Edinburgh and all efforts were made to minimize animal suffering.
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