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Biotinylated anti mouse or anti rabbit antibodies

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Biotinylated anti-mouse or anti-rabbit antibodies are secondary antibodies that have been conjugated with biotin, a small molecule that can bind to streptavidin or avidin. These antibodies are commonly used in various immunoassay techniques, such as Western blotting, ELISA, and immunohistochemistry, to detect and amplify the signal from primary antibodies that recognize mouse or rabbit antigens.

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Lab products found in correlation

Abc kit, by Vector Laboratories (4 mentions) Glial fibrillary acidic protein (gfap), by Agilent Technologies (3 mentions) Anti cd68, by Bio-Rad (2 mentions) Rabbit anti bdnf, by Santa Cruz Biotechnology (1 mentions) Mouse anti dcx, by Santa Cruz Biotechnology (1 mentions) Mab377, by Merck Group (1 mentions) Mab5258, by Merck Group (1 mentions)

Spelling variants of this product

Biotinylated antibodies (8 citations) Anti-mouse antibodies (2 citations) Biotinylated anti-mouse or anti-rabbit IgG antibodies (2 citations) Biotinylated anti-mouse IgG or anti-rabbit IgG secondary antibodies (2 citations)

4 protocols using biotinylated anti mouse or anti rabbit antibodies

1

Immunostaining of Neuroblasts, proBDNF, and BDNF

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To examine the effect of tPA on neuroblasts, proBDNF and BDNF, immunostaining was performed in five brain coronal sections and cervical spinal cord transverse sections. Briefly, 6-µm paraffin-embedded sections were deparaffinized and rehydrated. Antigen retrieval was performed by boiling sections in 10-mM citrate buffer (pH 6.0) for 10 min. After washing with PBS, sections were incubated with 0.3% H2O2 in PBS for 10 min, blocked with 1% BSA containing 0.3% Triton-X 100 for 1 h at room temperature, and incubated with mouse anti-DCX (1∶200; Santa Cruz Biotechnology, Santa Cruz, CA) or rabbit anti-proBDNF (1∶200; AbCam, Cambridge, MA) or rabbit anti-BDNF (1∶200; Santa Cruz Biotechnology, CA) at 4°C overnight. For negative controls, primary antibodies were omitted. After washing, sections were incubated with biotinylated anti-mouse or anti-rabbit antibodies (1∶200; Vector Laboratories, Inc., Burlingame, CA) for 30 min at room temperature. After an additional washing, sections were incubated with an avidin-biotin-peroxidase system (ABC kit, Vector Laboratories, Inc.), visualized with diaminobenzidine (Sigma, St. Louis, MO), and counterstained with hematoxylin.
Meng Y., Chopp M., Zhang Y., Liu Z., An A., Mahmood A, & Xiong Y. (2014). Subacute Intranasal Administration of Tissue Plasminogen Activator Promotes Neuroplasticity and Improves Functional Recovery following Traumatic Brain Injury in Rats. PLoS ONE, 9(9), e106238.
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2

Immunohistochemical Staining Procedure

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Antigen retrieval was performed by boiling sections in 10 mM citrate buffer (pH 6) for 10 minutes. After washing with PBS, sections were incubated with 0.3% H2O2 in PBS for 10 minutes, blocked with 1% bovine serum albumin containing 0.3% Triton X-100 at room temperature for 1 hour, and incubated with either anti–endothelial barrier antigen (EBA, 1:1000; Covance), anti-CD68 (1:200; AbD, Serotec, Kidlington, UK) or anti–glial fibrillary acidic protein (GFAP, 1:1000; Dako) or anti-NeuN monoclonal antibody (1:300, MAB 377, Chemicon) or anti-synaptophysin antibody (1:800; MAB5258, Millipore) or anti-fibrin antibody (1:1000, Acc Chem) at 4°C overnight. For negative controls, primary antibodies were omitted. After washing, sections were incubated with biotinylated anti–mouse or anti–rabbit antibodies (1:200; Vector Laboratories, Inc.) at room temperature for 30 minutes. After an additional washing, sections were incubated with an avidin-biotin peroxidase system (ABC kit; Vector Laboratories, Inc.), visualized with diaminobenzidine (Sigma), and counterstained with hematoxylin.
Zhang Y., Zhang Z.G., Chopp M., Meng Y., Zhang L., Mahmood A, & Xiong Y. (2016). Treatment of traumatic brain injury in rats with N-acetyl-seryl-aspartyl-lysyl-proline. Journal of neurosurgery, 126(3), 782-795.
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3

Immunohistochemical Staining of Brain Tissue

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Antigen retrieval was performed by boiling brain sections in 10 mM citrate buffer (pH 6.0) for 10 minutes. After washing with PBS, sections were incubated with 0.3 % H2O2 in PBS for 10 minutes, blocked with 1% BSA containing 0.3 % Triton-X 100 at room temperature for 1 hour, and incubated with anti-endothelial barrier antigen (EBA, 1:1000; COVANCE, CA) or anti-CD68 (1:200; Serotec, Kidlington, UK) or anti-glial fibrillary acidic protein (GFAP, 1:1000; Dako, Denmark) at 4°C overnight. For negative controls, primary antibodies were omitted. After washing, sections were incubated with biotinylated anti-mouse or anti-rabbit antibodies (1:200; Vector Laboratories, Inc.) at room temperature for 30 minutes. After an additional washing, sections were incubated with an avidin-biotin-peroxidase system (ABC kit, Vector Laboratories, Inc.), visualized with diaminobenzidine (Sigma), and counterstained with hematoxylin.
Zhang Y., Chopp M., Zhang Z.G., Katakowski M., Xin H., Qu C., Ali M., Mahmood A, & Xiong Y. (2016). Systemic administration of cell-free exosomes generated by human bone marrow derived mesenchymal stem cells cultured under 2D and 3D conditions improves functional recovery in rats after traumatic brain injury. Neurochemistry international, 111, 69-81.
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4

Immunohistochemical Staining of Brain Tissue

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Antigen retrieval was performed by boiling sections in 10 mM citrate buffer (pH 6.0) for 10 minutes. After washing with PBS, sections were incubated with 0.3 % H2O2 in PBS for 10 minutes, blocked with 1% BSA containing 0.3 % Triton-X 100 at room temperature for 1 hour, and incubated with mouse anti-doublecortin (1:200; DCX, Santa Cruz Biotechnology, Santa Cruz, CA) or anti-endothelial barrier antigen (EBA, 1:1000; COVANCE, CA) or anti-CD68 (1:200; Serotec, Kidlington, UK) or anti-glial fibrillary acidic protein (GFAP, 1:1000; Dako, Denmark) at 4°C overnight. For negative controls, primary antibodies were omitted. After washing, sections were incubated with biotinylated anti-mouse or anti-rabbit antibodies (1:200; Vector Laboratories, Inc.) at room temperature for 30 minutes. After an additional washing, sections were incubated with an avidin-biotin-peroxidase system (ABC kit, Vector Laboratories, Inc.), visualized with diaminobenzidine (Sigma), and counterstained with hematoxylin.
Zhang Y., Chopp M., Meng Y., Katakowski M., Xin H., Mahmood A, & Xiong Y. (2015). Effect of exosomes derived from multipluripotent mesenchymal stromal cells on functional recovery and neurovascular plasticity in rats after traumatic brain injury. Journal of neurosurgery, 122(4), 856-867.
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