The human gastric cancer cell line MGC803 (BNCC100665) was purchased from the BeNa Culture Collection and cultured in RPMI-1640 medium (Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS; cat. no. 26140079; Gibco; Thermo Fisher Scientific, Inc.), at 37°C in a humidified atmosphere (5% CO2). MGC803/DDP cells with induced resistance to cisplatin (DDP; cat. no. 15663-27-1; Sigma-Aldrich; Merck KGaA) were obtained by exposing MGC803 cells to a concentration gradient of DDP as previously described (23 (link)).
Mgc803
Manufactured by Merck Group
Sourced in United States
The MGC803 is a laboratory equipment product developed by Merck Group. It is designed to perform various scientific tasks and experiments within a controlled laboratory environment. The core function of the MGC803 is to provide a reliable and efficient platform for conducting a range of analytical and research activities.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Mgc 803, by BNCC (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Mgc 803, by National Collection of Authenticated Cell Cultures (1 mentions)
Paclitaxel, by Merck Group (1 mentions)
Ges 1, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Hgc 27, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Hgc 27, by National Collection of Authenticated Cell Cultures (1 mentions)
Ham s f 12 medium, by Procell (1 mentions)
Xav939, by Merck Group (1 mentions)
Lly 507, by Merck Group (1 mentions)
Mkn 45, by BNCC (1 mentions)
Mkn 45, by Merck Group (1 mentions)
Cisplatin, by Merck Group (1 mentions)
7 protocols using mgc803
1
Establishing Cisplatin-Resistant Gastric Cancer Cells
2
Establishment of Paclitaxel-Resistant Gastric Cancer Cells
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The human gastric cancer cell line MGC-803 was obtained from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientifics, Inc.) and incubated at 37°C in a humidified incubator with 5% CO2. paclitaxel-resistant MGC-803R cells were established by continuous exposure to stepwise-increasing concentrations of paclitaxel (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). MGC-803 cells were initially cultured in DMEM containing a low concentration of paclitaxel (1 µg/l). The cells were then sub-cultured every 2 weeks in DMEM with increasing concentrations of the drug, a 25% increase each time. Finally, cells that were viable in the cell culture medium with a high concentration of paclitaxel (100 µg/l) were designated as paclitaxel-resistant MGC-803R cells. These cells were maintained in the drug-containing medium following induction, but were cultured in drug-free medium for 1 week at 37°C prior to subsequent experimentation. Parental cells, denoted as MGC-803S, were cultured under the same conditions without treatment.
3
Cell Culture Conditions for Gastric Cancer
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The human GC cell lines HGC27 and AGS were purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China), and the human GC cell line MGC803 and human gastric epithelium cell line GES-1 were obtained from the Key Laboratory for Tumor Precision Medicine of Shaanxi Province (Xi’an, China). HGC27 and MGC803 cells were cultured in the RPMI 1640 medium (Sigma-Aldrich, WI, USA), AGS cells were maintained in Ham’s/F-12 medium (Procell, Wuhan, China), and GES-1 cells were grown in Eagle’s minimum essential medium (DMEM, Sigma-Aldrich, WI, USA), containing 10% fetal bovine serum (Biological Industries, Israel) and 1% penicillin/streptomycin (New Cell & Molecular Biotech, Suzhou, China). All cells were incubated in a water-saturated atmosphere of 5% CO2 at 37 °C and were collected at the peak of the logarithmic growth phase for experiments.
4
Gastric Cancer Cell Line Culture and Inhibitor Treatment
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Human GC cell lines MGC-803, BGC-823, MKN-45, AGS, and SGC-7901, and the human gastric mucosal epithelial cell line GES-1 were all purchased from the Cell Bank of Shanghai Institute of Cell Biology (Shanghai, P.R. China). They were cultured in Roswell Park Memorial Institute (RPMI-1640) culture medium (HyClone, Irvine, CA, USA) with 10% fetal bovine serum (FBS; HyClone) and maintained at 37°C in 5% CO2 and passaged at 80%–90% confluency every 3 or 4 days15 (link).
MGC-803 cells were pretreated with 5 μmol/L of LLY-507 (inhibitor of SMYD; Sigma-Aldrich, St. Louis, MO, USA) or 10 μmol/L of XAV939 (inhibitor of β-catenin; Sigma-Aldrich) for 1 h and then cultured as before for detection of inhibitor effects.
MGC-803 cells were pretreated with 5 μmol/L of LLY-507 (inhibitor of SMYD; Sigma-Aldrich, St. Louis, MO, USA) or 10 μmol/L of XAV939 (inhibitor of β-catenin; Sigma-Aldrich) for 1 h and then cultured as before for detection of inhibitor effects.
5
Establishment of Cisplatin-Resistant Gastric Cancer Cell Lines
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Human GC cell lines (MGC-803 and MKN-45) were brought from Bena Culture Collection (Beijing, China). Both cell lines were maintained in RPMI 1640 (HyClone, Logan, UT, USA) with 10% (v/v) fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA), penicillin (100 U/mL) and streptomycin (100 mg/mL) (Invitrogen, Carlsbad, CA, USA). Continuous exposure to stepwise-enhancing concentrations of cisplatin (Sigma-Aldrich, St. Louis, MO, USA) was used to establish cisplatin-resistant MGC-803 and MKN-45 cells. These cells were initially incubated in medium with a relatively low concentration of cisplatin (1 µM) in medium for 4 weeks. Subsequently, the surviving cells were exposed to a middle concentration of cisplatin (2 µM) for 6 weeks. Lastly, MGC-803 and MKN-45 cells were incubated in the culture medium containing a high concentration of cisplatin (5 µM). All cells were grown in a moist atmosphere with 5% carbon dioxide incubator at 37°C.
6
Gastric Cancer Cell Line Transfection
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Human gastric cancer cell lines MGC‐803 and SGC‐7901 cells were obtained from American Type Culture Collection (ATCC) and cultured in RPMI 1640 (Gibco) containing 10% fatal bovine serum (FBS) at 37°C in a humidified incubator of 5% CO2. Lipofectamine 2000 (11668019, Invitrogen) was utilized for transfection of the shRNA plasmids into MGC‐803 and SGC‐7901 cells. Then, MGC‐803 cells were cultured with 1 mg/mL puromycin (Sigma) to select stably transfected ANLN shRNA cells and used for the animal assays.
7
Establishing Cisplatin-Resistant Gastric Cancer Cell Lines
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Cell culture GC cell lines MGC803 and MKN45 were obtained from the ATCC. They have been authenticated by a STR(Short TandemRepeat) DNA pro ling analysis and routinely examined for Mycoplasma contamination. Both cell lines were maintained in RPMI 1640(HyClone, Logan, UT, USA) with 10% fetal bovine serum (FBS; Thermo Fisher Scienti c, Waltham, MA, USA), penicillin (100 U/mL) and streptomycin (100 mg/mL) (Invitrogen, Carlsbad, CA, USA). Continuous exposure to stepwise-enhancing concentrations of DDP (Sigma-Aldrich, St. Louis, MO, USA) was used to establish DDP resistant MGC803(MGC803/DDP) and MKN45(MKN45/DDP) cells. Brie y, these cells were initially cultured in medium with a relatively low concentration of DDP (0.1 µM) for 6 weeks. Subsequently, the surviving cells were exposed to higher concentration of DDP (0.2, 0.4, 0.8, 1.6 µM) for 6 weeks gradually. Lastly, MGC-803 and MKN-45 cells were incubated in the culture medium containing a high concentration of DDP (3.0 µM). All cells were grown in a moist atmosphere with 5% carbon dioxide incubator at 37 °C.
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