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3 amino 9 ethylcarbazole aec substrate solution

Manufactured by Vector Laboratories
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3-amino-9-ethylcarbazole (AEC) substrate solution is a laboratory reagent used as a chromogenic substrate in immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) applications. It produces a reddish-brown color when oxidized, allowing for the visualization of target analytes in biological samples.

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Lab products found in correlation

Goat anti rat igg secondary ab, by Southern Biotech (2 mentions)

Close spelling variants of this product

3-amino-9-ethylcarbazole (AEC) substrate 3-amino-9-ethylcarbazole (AEC) 3-amino-9-ethylcarbazole AEC substrate solution AEC substrate AEC solution

2 protocols using 3 amino 9 ethylcarbazole aec substrate solution

1

Epidermal Thickness and Immune Cell Analysis

Cited 1 time
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Skin sections were stained with H&E and the epidermal thickness was determined by measuring the average interfollicular distance under the microscope in a blinded manner. For IHC staining, skin cryosections were fixed, blocked, and stained with rat-anti-mouse Gr-1 mAb (1:50) followed by goat-anti-rat IgG secondary Ab (1:200, Southern Biotech) 4 (link). Slides were developed with 3-amino-9-ethylcarbazole (AEC) substrate solution (Vector Laboratories) and counterstained with hematoxylin. Images were acquired at x200 magnification using Aperio ScanScope digital scanners.
Cai Y., Xue F., Fleming C., Yang J., Ding C., Ma Y., Liu M., Zhang H.G., Zheng J., Xiong N, & Yan J. (2014). Differential Developmental Requirement and Peripheral Regulation for Dermal Vγ4 and Vγ6T17 Cells in Health and Inflammation. Nature communications, 5, 3986.
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2

Epidermal Thickness and Immune Cell Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Skin sections were stained with H&E and the epidermal thickness was determined by measuring the average interfollicular distance under the microscope in a blinded manner. For IHC staining, skin cryosections were fixed, blocked, and stained with rat-anti-mouse Gr-1 mAb (1:50) followed by goat-anti-rat IgG secondary Ab (1:200, Southern Biotech) 4 (link). Slides were developed with 3-amino-9-ethylcarbazole (AEC) substrate solution (Vector Laboratories) and counterstained with hematoxylin. Images were acquired at x200 magnification using Aperio ScanScope digital scanners.
Cai Y., Xue F., Fleming C., Yang J., Ding C., Ma Y., Liu M., Zhang H.G., Zheng J., Xiong N, & Yan J. (2014). Differential Developmental Requirement and Peripheral Regulation for Dermal Vγ4 and Vγ6T17 Cells in Health and Inflammation. Nature communications, 5, 3986.
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