Surface staining of BMC and spleen cells previously treated with erythrocyte lysis buffer (Qiagen) was performed with antibodies from BD Pharmingen against CD3-PE-Cy7, CD4-APC, CD8-PB, B220-APC, Ter119-PE-Cy7, CD11b-PE-Cy7, CD19-PE-Cy7, Gr-1-PE-Cy7, Gr-1-PB and Sca1-PB; cKit-APC from BioLegend (San Diego, CA, USA), and CD90.2-PE-Cy7 from eBioscience (San Diego, CA, USA) and measured using the CyanADP flow cytometer (Beckmann Coulter). Flow cytometry data were analyzed using the FlowJo 7.6 software (Tree Star, Inc., Ashland, OR, USA).
Sca1 pb
Manufactured by BioLegend
Sourced in United States, United Kingdom
Sca1-PB is a fluorescently-labeled antibody that binds to the Sca1 (Stem Cell Antigen-1) surface marker. Sca1 is commonly used to identify and isolate hematopoietic stem and progenitor cells in murine samples.
Automatically generated - may contain errors
Lab products found in correlation
Cd3 pe cy7, by BD (1 mentions)
Cd4 apc, by BioLegend (1 mentions)
B220 apc, by BioLegend (1 mentions)
C kit apc, by BioLegend (1 mentions)
Cd19 pe cy7, by BioLegend (1 mentions)
Cd11b pe cy7, by BioLegend (1 mentions)
Erythrocyte lysis buffer, by Qiagen (1 mentions)
Cyan adp flow cytometer, by Beckman Coulter (1 mentions)
Ter119 pe cy7, by BioLegend (1 mentions)
Gr1 pe cy7, by BioLegend (1 mentions)
Gr1 pb, by BioLegend (1 mentions)
Cd8 pb, by BioLegend (1 mentions)
Streptavidin apc, by Thermo Fisher Scientific (1 mentions)
Click it edu, by Thermo Fisher Scientific (1 mentions)
Mitotracker deep red, by Thermo Fisher Scientific (1 mentions)
Cd34 apc, by Thermo Fisher Scientific (1 mentions)
Gr 1 pe, by BioLegend (1 mentions)
2 protocols using sca1 pb
1
Surface Marker Profiling of Immune Cells
2
Flow Cytometry Profiling of Hematopoietic Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Suppliers of antibodies and dyes were as follows: eBioscience (Hatfield, Herts, UK): CD71-biotin, Ter119-PE, CD48-APC, CD150-PECy7, CD34-APC, FcγRII/III-PECy7, IL7Rα-PECy7, Flk3-PE, CD44-biotin, streptavidin-APC; Life Technologies (Paisley, Renf, UK): biotinylated lineage cocktail (MLM15), c-kit-APC-Cy7, Mac1-biotin, Gr1-PE; BioLegend (London, UK): Sca-1-PB. Mitochondria were stained with 500 nm Mitotracker DeepRed (Invitrogen) for 30 min at 37 °C in complete media. For cell cycle analysis, Click-IT EdU was used (Invitrogen) or for primary cells, 3 × 105 cells were sorted, fixed in 70% ethanol, and stained in 1× PBS, 50 µg/ml PI, 50 µg/ml RNase A.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!