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Dnmt1 elisa kit

Manufactured by Epigentek
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Sourced in United States

The DNMT1 ELISA kit is a quantitative in vitro enzyme-linked immunosorbent assay (ELISA) designed for the detection and quantification of DNMT1 (DNA Methyltransferase 1) levels in cell and tissue lysates.

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Lab products found in correlation

Multiskan ex microplate reader, by Thermo Fisher Scientific (1 mentions) Bca assay, by Merck Group (1 mentions)

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DNMT1

2 protocols using dnmt1 elisa kit

1

Quantifying DNMT1 Activity by ELISA

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Nuclear extract was carried out according to the manufacturer’s instruction (Beyotime Institute of Biotechnology, Shanghai, China). The activity of DNMT1 was detected by human DNA methyltransferase 1 (DNMT1) ELISA kit according to the manufacturer’s instructions(Epigentek, USA). The concentration of DNMT1 in samples were calculated by the working standard curve.
Xu X., Ji S., Chen Y., Xia S., Li Y., Chen L., Li Y., Zhang F., Zhang Z, & Zheng S. (2023). Induction of DNMT1-dependent demethylation of SHP-1 by the natural flavonoid compound Baicalein overcame Imatinib-resistance in CML CD34+ cells. Cell Communication and Signaling : CCS, 21, 47.
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2

Quantification of DNMT1 Protein via BCA and ELISA

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Total protein estimation using the bichoninic acid (BCA) assay (Sigma-Aldrich) was performed on 20 μL cell lysate according to the manufacturer’s instructions. Western blotting with fluorescence visualization was performed as described in [54 (link)].
Purified GFP-tagged DNMT1 protein bound to beads was quantified using a DNMT1 ELISA kit (Epigentek Cat #P-3011). Standards were prepared from 7.5, 10, 12.5 and 15 ng of purified DNMT1 (supplied in the kit), and absorbance was read at 450 and 620 nm on a Multiskan EX microplate reader (Thermo Electron).
Dagar V., Hutchison W., Muscat A., Krishnan A., Hoke D., Buckle A., Siswara P., Amor D.J., Mann J., Pinner J., Colley A., Wilson M., Sachdev R., McGillivray G., Edwards M., Kirk E., Collins F., Jones K., Taylor J., Hayes I., Thompson E., Barnett C., Haan E., Freckmann M.L., Turner A., White S., Kamien B., Ma A., Mackenzie F., Baynam G., Kiraly-Borri C., Field M., Dudding-Byth T, & Algar E.M. (2018). Genetic variation affecting DNA methylation and the human imprinting disorder, Beckwith-Wiedemann syndrome. Clinical Epigenetics, 10, 114.
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