Cells were stained for 30 min on ice with the following mouse anti-human antibodies: CD3-BrilliantViolet421, CD4-AlexaFluor700, CD8β-PE, CD31-FITC, CD34-PE, CD45RA-PE/CF594, TCRgd-FITC (BD Biosciences), CD5-PE/Cy7, CD7-AlexaFluor700, CD45-APC/eFluor780, TCRab-APC (eBiosciences), CD8α-PE/Dazzle, CD38-BrilliantViolet421 (BioLegend). Cells were resuspended in flow cytometry buffer containing DAPI, data was collected using LSR Fortessa flow cytometer (BD Biosciences) and analyzed using FlowJo version 9.7.6. For intracellular staining, cells were fixed and permeabilized using Fixation/Permeabilization kit with GolgiPlug™ (BD Biosciences) as per manufacturer’s instructions.
Cd38 brilliant violet 421
Manufactured by BioLegend
CD38-Brilliant Violet 421 is a laboratory reagent that can be used to detect and analyze the expression of the CD38 cell surface marker. It is a fluorescently-labeled antibody that binds to the CD38 antigen, allowing for the identification and quantification of CD38-positive cells in various samples.
Automatically generated - may contain errors
Lab products found in correlation
Cd4 percp cy5, by BioLegend (4 mentions)
Cd25 pe, by BioLegend (4 mentions)
Cd3 fitc, by BioLegend (4 mentions)
Cd8 brilliant violet 510, by BioLegend (4 mentions)
Hla dr apc fire 750, by BioLegend (4 mentions)
Cobas amplicor, by Roche (4 mentions)
Cd57 allophycocyanin apc, by BioLegend (3 mentions)
Facscanto 2, by BD (2 mentions)
Cd34 pe, by BD (1 mentions)
Cd45 apc efluor780, by Thermo Fisher Scientific (1 mentions)
Cd4 alexafluor700, by BD (1 mentions)
Cd31 fitc, by BD (1 mentions)
Cd5 pe cy7, by Thermo Fisher Scientific (1 mentions)
Cd7 alexa fluor 700, by Thermo Fisher Scientific (1 mentions)
Cd8β pe, by BD (1 mentions)
Cd45ra pe cf594, by BD (1 mentions)
Tcrgd fitc, by BD (1 mentions)
Lsrfortessa flow cytometer, by BD (1 mentions)
Golgiplug, by BD (1 mentions)
Human soluble cd14 scd14 elisa kit, by MultiSciences Biotech (1 mentions)
5 protocols using cd38 brilliant violet 421
1
Multiparametric Flow Cytometry Immunophenotyping
2
Quantifying Immune Activation Markers in HIV
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Sera samples of immunological responders and non-responders were collected to measure the bacterial translocation markers. Following the standard protocols, human Lipopolysaccharides (LPS) ELISA Kit (CUSABIO; Wuhan, China) and Human soluble CD14 (sCD14) ELISA Kit (MultiSciences, Hangzhou, China) were used to test plasma LPS and sCD14. Flow cytometry and Cobas Amplicor (Roche Molecular Systems Inc., Branchburg, New Jersey, United States) were used to quantify CD4 + /CD8 + T-cells and HIV-1 RNA, respectively. Fresh anticoagulated whole blood was used to quantify the expressing markers of immune activation (CD25 +, CD38 +, HLADR +, or CD38 + /HLA-DR +) of CD4 + and CD8 + T-cells and immune senescence (CD57 +) by BD FACS Canto II flow cytometer (BD Biosciences, California, United States). The antibodies needed during the experiment were purchased from Biolegend (San Diego, CA), including CD3-FITC, CD4- PerCP/Cy5.5, CD8-Brilliant Violet 510™, CD38-Brilliant Violet 421, CD25-PE, HLA-DR-APC/Fire™ 750, and CD57-allophycocyanin (APC).
3
Quantification of T-cell Markers in HIV
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Quantifications of CD4+ and CD8+ T-cells as well as HIV-1 RNA were carried out in HIV-infected individuals using flow cytometry and Cobas Amplicor (Roche Molecular Systems Inc., Branchburg, New Jersey, USA) as the clinical routine. The percentage of CD4+ and CD8+ T cells expressing markers of activation (CD25+, CD38+, HLADR+, or CD38+/HLA-DR+) and senescence (CD57+) were quantified by the BD FACS Canto II flow cytometer (BD Biosciences, California, USA) using fresh anticoagulated whole blood. Antibody such as CD3-FITC, CD4- PerCP/Cy5.5, CD8-Brilliant Violet 510™, CD38-Brilliant Violet 421, CD25-PE, HLA-DR-APC/Fire™ 750, and CD57-allophycocyanin (APC) were purchased from Biolegend (San Diego, CA).
4
Immunophenotyping of HIV Infection
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Quanti cations of and CD8+ as well as HIV-1 RNA were carried out in HIV-infected individuals using ow cytometry and Cobas Amplicor (Roche Molecular Systems Inc., Branchburg, New Jersey, USA) as the clinical routine. The percentage of CD4+ and CD8+ T cells expressing markers of activation (CD25+, CD38+, HLADR+, or CD38+/HLA-DR+) and senescence (CD57+) were quanti ed by the BD FACS Canto II ow cytometer (BD Biosciences, California, USA) using fresh anticoagulated whole blood. Antibody such as CD3-FITC, CD4-PerCP/Cy5.5, CD8-Brilliant Violet 510™, CD38-Brilliant Violet 421, CD25-PE, HLA-DR-APC/Fire™ 750, and CD57-allophycocyanin (APC) were purchased from Biolegend (San Diego, CA).
5
Quantification of T-cell Subsets in HIV
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Quanti cations of CD4+ and CD8+ T-cells as well as HIV-1 RNA were carried out in HIV-infected individuals using ow cytometry and Cobas Amplicor (Roche Molecular Systems Inc., Branchburg, New Jersey, USA) as the clinical routine. The percentage of CD4+ and CD8+ T cells expressing markers of activation (CD25+, CD38+, HLADR+, or CD38+/HLA-DR+) and senescence (CD57+) were quanti ed by the BD FACS Canto II ow cytometer (BD Biosciences, California, USA) using fresh anticoagulated whole blood. Antibody such as CD3-FITC, CD4-PerCP/Cy5.5, CD8-Brilliant Violet 510™, CD38-Brilliant Violet 421, CD25-PE, HLA-DR-APC/Fire™ 750, and CD57-allophycocyanin (APC) were purchased from Biolegend (San Diego, CA).
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