Rabbit anti atg3

The following primary antibodies were used for immunofluorescence (IF) or immunoblotting (IB): rabbit anti-ATG3 (Abcam, Cambridge, UK; ab108251; IF, 1:50; IB, 1:1000), anti-ATG4B (CST, Leiden, The Netherlands; 5299; IF, 1:100; IB, 1:1000), anti-ATG7 (Abcam, ab133528; IB, 1:1000), anti-SQSTM1 (Abcam, ab109012; IF, 1:200; IB, 1:20,000), anti-OPTN (Abcam, ab23666; IF, 1:50; IB, 1:1000), anti-CACO2/NDP52 (Abcam, ab68588; IF, 1:100; IB, 1:2000), anti-LC3B (CST, 2775; IB, 1:1000), anti-TUBA1A (Santa Cruz Biotechnology, Heidelberg, Germany; SC-5546; IB, 1:1000), anti-USP30 (Abbkine Scientific, Wuhan, China; ABP52679; IB, 1:1000), anti-DNM1L (Cusabio, Houston, TX, USA; CSB-PA002203; IB, 1:1000), mouse anti-PEX14 (IF, 1:100) [40 (link)], and anti-actin (Sigma, A5316; IB, 1:10,000). The secondary antibodies for IF were conjugated to Alexa Fluor 488 (Invitrogen, A11017; 1:2000) or Texas Red (Calbiochem, Darmstadt, Germany; 401355; 1:200), and the secondary antibodies for IB were conjugated to alkaline phosphatase (Sigma, A3687 and A2429; 1:5000 and 1:10,000, respectively).

Primary antibodies were as follows: mouse anti-a2V (Covance, Denver, PA); rabbit anti-GAPDH (Cell signaling, Danvers, MA); rat anti-F4/80, rabbit anti-LC3B, rabbit anti-LAMP-1, rat anti-LAMP-2, rabbit anti-ATG3, rabbit anti-ATG4, rabbit anti-ATG7, rabbit anti-NF-κB p65 (all from Abcam). Secondary antibodies were as follows: goat anti-rabbit IgG-FITC, -mouse IgG AF-594, -rabbit IgG AF-594 (Invitrogen), rabbit anti-rat IgG-FITC (Abcam), goat anti-rabbit, -rat, -mouse IgG-HRP (Santa Cruz Biotechnology), donkey anti-rabbit IRDye-800CW (LI-COR Bioscience, Lincoln, NE) and EnVision + dual link System-Horseradish peroxidase (HRP) (Dako). Isotype control antibodies were as follows: control mouse IgG (R&D Systems); rat IgG isotype, mouse IgG isotype and rabbit IgG isotype (Abcam).