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Media recorder 4

Manufactured by Noldus

The Media Recorder 4.0 is a professional-grade video recording system designed for laboratory environments. It captures high-quality video and audio from a variety of sources, enabling users to document and analyze research activities. The device features adjustable video and audio settings, allowing for customization to meet specific needs.

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Lab products found in correlation

3 protocols using media recorder 4

1

Behavioral Scoring of Pavlovian Conditioning Sessions

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Behavior during Pavlovian conditioning sessions was video recorded (Media Recorder 4.0, Noldus) using cameras positioned a standardized distance behind each chamber. Videos from sessions 1, 4, 8 and 12 were scored offline by observers who were blind to the identity and anatomical target group of the rats. Each cue (7-sec, 25 per session) and laser (1 or 5-sec, 25 per session) event was scored for the occurrence and onset latency of the following behaviors. Locomotion: Defined as the rat moving all four feet in a forward direction (i.e., not simply lifting feet in place). Cue Approach: Defined as the rat’s nose coming within 1 in of the cue light (trials in which the rat’s nose was in front of the light when it was presented were not counted in the approach measure). Approach often involved the rat moving from another area of the chamber to come in physical contact with the cue light while it was illuminated. Rearing: Defined as the rat lifting its head and front feet off the chamber floor, either onto the side of the chamber, or into the air. Rotation: Defined as the rat making a complete 360-degree turn in one direction.
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2

Behavioral Scoring of Pavlovian Conditioning Sessions

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Behavior during Pavlovian conditioning sessions was video recorded (Media Recorder 4.0, Noldus) using cameras positioned a standardized distance behind each chamber. Videos from sessions 1, 4, 8 and 12 were scored offline by observers who were blind to the identity and anatomical target group of the rats. Each cue (7-sec, 25 per session) and laser (1 or 5-sec, 25 per session) event was scored for the occurrence and onset latency of the following behaviors. Locomotion: Defined as the rat moving all four feet in a forward direction (i.e., not simply lifting feet in place). Cue Approach: Defined as the rat’s nose coming within 1 in of the cue light (trials in which the rat’s nose was in front of the light when it was presented were not counted in the approach measure). Approach often involved the rat moving from another area of the chamber to come in physical contact with the cue light while it was illuminated. Rearing: Defined as the rat lifting its head and front feet off the chamber floor, either onto the side of the chamber, or into the air. Rotation: Defined as the rat making a complete 360-degree turn in one direction.
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3

Evaluating Pesticide Toxicity in Zebrafish

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Mortality as evidenced by egg coagulation or cardiac arrest was determined at 24, 48, 72, and 96 hpf. At the same time, the rate of deformity (including pericardial edema, yolk sac edema, and tail flicks) was calculated for each treatment group at 48, 72, and 96 hpf, the temperature was kept at 28 ± 2 °C during all measurements. The hatching rate, defined as total separation of juvenile fish from the egg membrane, was calculated at 48 hpf. Five embryos in one replicate were selected and recorded under a stereoscopic microscope for 1 min tail flicks at 24 hpf and for 30-s heart rate at 48 hpf using MediaRecorder4.0 (Noldus, Wageningen, The Netherlands). We considered a tail flick as a movement more significant than 15 pixels recorded by the software. The heart rate values were multiplied by 2 and converted to beats per minute. Video records were digitized for analysis using DanioScope 1.1 (Noldus, Wageningen, The Netherlands). The effects of pesticide exposure are expressed as10% benchmark dose levels (BMDL10 values). For tail flicks and heart beat measurements, only alive eggs or larvae were tested. The hatching rates were calculated by dividing the number of hatched eggs by the number of all the alive eggs and larvae.
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