Rats were sacrificed on the 1st, 14th, and 28th day after surgery. Three rats in each group were sacrificed on the 1st and 14th day, and four rats were sacrificed on the 28th day. Then, the tendon of the injured site was collected. For histological analysis, tendons were fixed in 4% paraformaldehyde for 24 h. Sagittal paraffin sections were prepared by embedding the samples in paraffin and cutting into 4 µm thick sections. Four sections per sample were stained with HE and observed under the OLYMPUS EX-51 microscope (Tokyo, Japan) at 100 × magnification. Masson’s trichrome stain (Solarbio, G1340) was performed according to kit directions. RECA-1 is a cell surface antigen expressed by rat endothelial cells. In this study, RECA-1 was selected for immunohistochemistry analysis on days 1, 14 and 28. Then, 5-μm-thick continuous sections were incubated with an anti-RECA-1 antibody (abcam, ab22492, 1:1000) produced in rabbit followed by goat anti-rabbit immunoglobulin antibody conjugated by horseradish peroxidase. Then, slides were visualized using diaminobenzidine (DAB) substrate.
Ex51 microscope
Manufactured by Olympus
Sourced in Japan
The EX51 microscope is a high-quality optical microscope designed for laboratory use. It features a binocular viewing head, a built-in illumination system, and a range of objective lenses for magnification. The EX51 is suitable for a variety of applications requiring detailed observation and analysis of samples.
Automatically generated - may contain errors
Lab products found in correlation
Ab22492, by Abcam (1 mentions)
Masson s trichrome stain, by Solarbio (1 mentions)
Hematoxylin and eosin, by Vector Laboratories (1 mentions)
Prolong gold and dapi, by Thermo Fisher Scientific (1 mentions)
Vectastain abc, by Vector Laboratories (1 mentions)
Stellaris 8 falcon confocal microscope, by Leica (1 mentions)
A1 confocal microscope, by Leica (1 mentions)
Mhc 2, by Abcam (1 mentions)
N cadherin, by Santa Cruz Biotechnology (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Nf κb p65, by Santa Cruz Biotechnology (1 mentions)
Nf κb p50, by Santa Cruz Biotechnology (1 mentions)
Alexa fluor 594 secondary antibody, by Thermo Fisher Scientific (1 mentions)
Bcl 3, by Santa Cruz Biotechnology (1 mentions)
Cd11b, by Abcam (1 mentions)
Cd11b, by BD (1 mentions)
Live dead staining kit, by Thermo Fisher Scientific (1 mentions)
6 protocols using ex51 microscope
1
Rat Tendon Histological Analysis
2
Histological Analysis of RC Tendons
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For histological analysis, RC tendons were placed in 4% buffered formalin. Serial sagittal paraffin sections were prepared by embedding the samples in paraffin and cutting into 4-µm thick sections. Four sections per sample were stained with HE and observed under the OLYMPUS EX-51 microscope (Tokyo, Japan) at 100× magnifications.
3
Immunohistochemical Analysis of Mouse Thyroid
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse thyroids were quickly dissected and immersion-fixed in 10% formalin, paraffin-embedded, sectioned, and stained with hematoxylin and eosin (Vector Laboratories). For immunofluorescence, thyroid sections (6 μm) were deparaffinized in Citrisolv, then rehydrated using a graded ethanol series, followed by antigen retrieval in citrate buffer, blocking in 1.5% goat serum, incubation with primary antibodies (overnight, 4°C) and Alexa Fluor–conjugated secondary antibodies (Invitrogen A11073, A11001, A21422, A21428, A21245; Jackson ImmunoResearch 712-606-153; 1 hour, room temperature), counterstaining with ProLong Gold and DAPI (Invitrogen), and imaging with Nikon A1 confocal microscope or Leica STELLARIS 8 FALCON confocal microscope. Quantification of CD45+ cells in the proportion of total cells was performed using AIVIA Artificial Intelligence-guided Software. Immunohistochemistry of Ki67 used VECTASTAIN-ABC (Vector Laboratories) with 40× objective image capture (Olympus EX51 Microscope). Quantitation of Ki67-positive nuclei as a fraction of total thyroid nuclei per field, or Mac2-immunostained cells as a fraction of total thyroid nuclei per field, was performed using Imaris software (version 7.7.2).
4
Invasive Diagnostics for GVHD Monitoring
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Simultaneous development of GVHD features led to invasive diagnostic procedures, where trunk punch biopsy was performed for patients with skin rash and colonoscopic biopsy was performed for patients with persistent diarrhea or hematochezia. BM biopsy was not routinely conducted due to its invasiveness despite occurrence of cytopenia. Biopsy specimens were examined after hematoxylin and eosin staining using an Olympus EX51 microscope (Olympus Life Science, Waltham, MA, USA).
5
Immunofluorescent Staining of Tissue Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Frozen or paraffin tissue sections were processed for Immunofluorescent staining. Non-specific binding sites were blocked by 2.5% goat serum in bovine serum albumin (0.5% w/v) for 1 hour. The sections were then incubated with the following antibodies for overnight at 4°C: GR-1 (BD Biosciences, 1:100); CD11b (BD Biosciences, 1:100); CD11b (Abcam, 1:100); CD16 (Abcam, 1:100); CD11c (Abcam, 1:100); CD4 (R&D system, 1:50); CD4 (BD Biosciences, 1:100); MHCII (Abcam, 1:100); FOXP3 (Abcam, 1:100); Vitamin D receptor (VDR; Abcam, 1:100) Bcl-3 (Santa Cruz, sc-13038, 1:100); NFκB-p50 (Santa Cruz, sc-114, 1:100); NFκB-p52 (Santa Cruz, sc-298, 1:100); NFκB-p65 (Santa Cruz, sc-109, 1:100); Gli1 (Santa Cruz, sc-20687, 1:100); Cyclin D1 (Neomarkers, RM-9104-s1, 1:100); N-cadherin (Santa Cruz, sc-7939, 1:100) and K17 (Neomarkers, MS-489-S1, 1:100). After washing 3 times with PBS, the sections were incubated with Alexa Fluor® 488 or Alexa Fluor® 594 secondary antibodies (Life Technologies) for 1 hour at room temperature. After removal of antibodies, the sections were rinsed with PBS and mounted with mounting medium containing DAPI. Fluorescence was immediately recorded on an Olympus EX51 microscope.
6
Tissue Viability Quantification via Live/Dead Stain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue viability was assessed using a commercial Live Dead staining kit (Molecular Probes, Invitrogen UK). Stained RTTFs were examined using an Olympus EX51 microscope (for cell counts, n=2 per group) with a 5x objective over a central Region of Interest (ROI; 1.7mm x 1.3mm).
Post image analysis and quantification of tissue viability (cell counts) was performed using Image J and a fascicle was defined as viable if 50% or more of the observed cells in the ROI were living.
Post image analysis and quantification of tissue viability (cell counts) was performed using Image J and a fascicle was defined as viable if 50% or more of the observed cells in the ROI were living.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!