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Rabbit anti notch1 antibody

Manufactured by Abcam
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Rabbit anti-NOTCH1 antibody is a primary antibody that specifically recognizes the NOTCH1 protein. NOTCH1 is a transmembrane receptor that plays a key role in cell signaling pathways involved in cell differentiation and development. This antibody can be used to detect and study the expression and localization of NOTCH1 in various experimental systems.

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Lab products found in correlation

Chip assay kit, by Merck Group (2 mentions) Pierce protein a g agarose, by Thermo Fisher Scientific (2 mentions) Rabbit anti beta catenin antibody, by Abcam (2 mentions) Rabbit anti muc2 antibody, by Proteintech (2 mentions) Rabbit anti lyz1 antibody, by Abcam (2 mentions) Rabbit anti chga antibody, by Abcam (2 mentions)

Close spelling variants of this product

Rabbit monoclonal anti-Notch1 (NICD) antibody Rabbit anti-Notch1 Anti-Notch1 antibody Notch1-antibody Anti-Notch1 Notch1 Rabbit anti-

4 protocols using rabbit anti notch1 antibody

1

NOTCH1 Binding to SNAI1 Promoter

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Chromatin from 5 × 106 RD cells was isolated and fixed with 1% formaldehyde, sonicated, and processed according to the manufacturer's protocols (ChIP Assay Kit, Millipore). Immunoprecipitation was performed using 5 mg rabbit anti-NOTCH1 antibody (Abcam) or rabbit immunoglobulin G (IgG) and Pierce Protein A/G Agarose (Thermo Scientific). The immunoprecipitated DNA was subjected to real-time PCR with primers that target the SNAI1 promoter with negative controls in a region 7.5 kb upstream and an ORF (open reading frame)-free region in chromosome 6. All signals were normalized against input by the percentage input calculation method and normalized to IgG signal. Significance was calculated by Student's t test.
Ignatius M.S., Hayes M.N., Lobbardi R., Chen E.Y., McCarthy K.M., Sreenivas P., Motala Z., Durbin A.D., Molodtsov A., Reeder S., Jin A., Sindiri S., Beleyea B.C., Bhere D., Alexander M.S., Shah K., Keller C., Linardic C.M., Nielsen P.G., Malkin D., Khan J, & Langenau D.M. (2017). The NOTCH1/SNAIL1/MEF2C Pathway Regulates Growth and Self-Renewal in Embryonal Rhabdomyosarcoma. Cell reports, 19(11), 2304-2318.
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2

NOTCH1 Binding to SNAI1 Promoter

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Chromatin from 5 × 106 RD cells was isolated and fixed with 1% formaldehyde, sonicated, and processed according to the manufacturer's protocols (ChIP Assay Kit, Millipore). Immunoprecipitation was performed using 5 mg rabbit anti-NOTCH1 antibody (Abcam) or rabbit immunoglobulin G (IgG) and Pierce Protein A/G Agarose (Thermo Scientific). The immunoprecipitated DNA was subjected to real-time PCR with primers that target the SNAI1 promoter with negative controls in a region 7.5 kb upstream and an ORF (open reading frame)-free region in chromosome 6. All signals were normalized against input by the percentage input calculation method and normalized to IgG signal. Significance was calculated by Student's t test.
Ignatius M.S., Hayes M.N., Lobbardi R., Chen E.Y., McCarthy K.M., Sreenivas P., Motala Z., Durbin A.D., Molodtsov A., Reeder S., Jin A., Sindiri S., Beleyea B.C., Bhere D., Alexander M.S., Shah K., Keller C., Linardic C.M., Nielsen P.G., Malkin D., Khan J, & Langenau D.M. (2017). The NOTCH1/SNAIL1/MEF2C Pathway Regulates Growth and Self-Renewal in Embryonal Rhabdomyosarcoma. Cell reports, 19(11), 2304-2318.
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3

Immunohistochemical Analysis of Intestinal Markers

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Para n sections (5-µm) of the ileum tissue underwent appropriate heat-induced antigen retrieval and blocked as previously described ( 16). The following primary antibodies were used: rabbit anti-Notch1 antibody (Abcam, Shanghai, China), rabbit anti-beta Catenin antibody (Abcam), rabbit anti-Muc2 antibody (Proteintech, Wuhan, China), rabbit anti-Lyz1 antibody (Abcam) and rabbit anti-Chga antibody (Abcam). For negative controls, mouse or rabbit nonimmune serum, instead of the primary Abs, was used to treat tissue sections. Intensities quanti cation of Notch1, β-catenin, Muc2, Lyz1 and Chga staining was performed by automated image analysis in ve randomly chosen 200X elds of each sample.
Deng F., Hu J., Yang X., Wang Y., Lin Z., Sun Q, & Liu K. (2020). Interleukin-10 expands transit-amplifying cells while depleting Lgr5+ stem cells via inhibition of Wnt and notch signaling. Biochemical and biophysical research communications, 533(4).
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4

Immunohistochemical Analysis of Intestinal Markers

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Para n sections (5-µm) of the ileum tissue underwent appropriate heat-induced antigen retrieval and blocked as previously described ( 16). The following primary antibodies were used: rabbit anti-Notch1 antibody (Abcam, Shanghai, China), rabbit anti-beta Catenin antibody (Abcam), rabbit anti-Muc2 antibody (Proteintech, Wuhan, China), rabbit anti-Lyz1 antibody (Abcam) and rabbit anti-Chga antibody (Abcam). For negative controls, mouse or rabbit nonimmune serum, instead of the primary Abs, was used to treat tissue sections. Intensities quanti cation of Notch1, β-catenin, Muc2, Lyz1 and Chga staining was performed by automated image analysis in ve randomly chosen 200X elds of each sample.
Deng F., Hu J., Yang X., Wang Y., & Liu K. (2020). Interleukin-10 Expands Transit-Amplifying Cells While Depleting Lgr5+ Stem Cells via Inhibition of Wnt and Notch Signaling.
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