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Accu check advantage blood glucose monitor

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The Accu-Chek Advantage Blood Glucose Monitor is a medical device used for measuring blood glucose levels. It is designed to provide users with accurate and reliable results to help manage their diabetes.

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Lab products found in correlation

Adiponectin, by ALPCO (1 mentions) Resistin, by BioVendor (1 mentions) Ultrasensitive rat insulin elisa, by Mercodia (1 mentions) Cobas integra 800 analyzer, by Roche (1 mentions) Leptin, by R&D Systems (1 mentions) Mouse albumin elisa kit, by Abcam (1 mentions) Cobas mira plus, by Roche (1 mentions)

7 protocols using accu check advantage blood glucose monitor

1

Fructose-Induced Metabolic Dysregulation

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Chow and water (with or without fructose) consumption were measured weekly. The total caloric intake was calculated using 2.89 kcal per gram of chow consumed and that each ingested gram of fructose corresponds to 4.0 kcal.
After 8 week of fructose overload, glucose tolerance test of all animals was performed after a 12-h fasting with a specific device (Accu-Check Advantage Blood Glucose Monitor, Roche Diagnostic Corporation, Indianapolis. IN). Animals received a dose of 1.5 g/kg body weight intraperitoneally (intraperitoneal glucose) and a drop of blood from the end of the tail was collected at baseline and 15, 30, 60, 90 and 120 minutes after the glucose injection [21 (link)]. Triglycerides and total cholesterol were analyzed in the serum by the chemiluminescent method (Lab test, Brazil).
Farah D., Nunes J., Sartori M., Dias D.D., Sirvente R., Silva M.B., Fiorino P., Morris M., Llesuy S., Farah V., Irigoyen M.C, & De Angelis K. (2016). Exercise Training Prevents Cardiovascular Derangements Induced by Fructose Overload in Developing Rats. PLoS ONE, 11(12), e0167291.
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2

Comprehensive Metabolic Profiling of Serum

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Blood samples were immediately centrifuged at 4000 g for 10 min at 4°C. These samples were aliquoted and applied for immediate assays of glucose, insulin, triglycerides, total cholesterol, creatinine, and adipocytokines. Serum glucose was measured using an Accu-Check Advantage Blood Glucose Monitor (Roche Diagnostic Corporation, Indianapolis, IN, USA). Serum insulin was measured using a Mercodia Ultrasensitive Rat Insulin ELISA (Mercodia AB, Uppsala, Sweden). Serum triglycerides, total cholesterol, and creatinine were determined through standard methods by using a COBAS Integra 800 analyzer (Roche Diagnostics, Indianapolis, IN). Serum adipocytokines, such as adiponectin (ALPCO Diagnostics, Salem, NH, USA), leptin (R&D Systems, Minneapolis, MN, USA), visfatin (Cayman Chemical, Ann Arbor, MI, USA), and resistin (BioVendor, Brno, Czech Republic), were measured using commercially available enzyme-linked immunosorbent assay kits.
Chou C.L., Lin H., Chen J.S, & Fang T.C. (2017). Renin inhibition improves metabolic syndrome, and reduces angiotensin II levels and oxidative stress in visceral fat tissues in fructose-fed rats. PLoS ONE, 12(7), e0180712.
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3

Diabetic db/db Mouse Model Characterization

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Adult male db/db mice (six-eight weeks old; Strain B6.BKS (D)-Leprdb/J, Stock No. 000697 B6 db) and age-matched non-diabetic lean control mice (littermate wild type) were included in the study. They were bred in the KSU animal care facility by mating heterozygous (Leprdb/+) males and females. Mice genotypes were confirmed by tail tissue and PCR analysis conducted by Transnetyx, Inc (Cordova, TN, USA). The mice were maintained at a room temperature of 22 °C ± 2 °C with a 12:12-h light/dark cycle and 40 to 60% humidity. They were housed in a specific pathogen-free environment, fed with standard rodent chow, and provided with water ad libitum. All experimental procedures were performed in accordance with the guidelines of the Institutional Research Ethics Committee (REC), King Saud University. To confirm the presence of diabetes in the models, the mice were made to fast for 6 h. Their blood glucose levels were then measured using 1–2 drops of blood collected from their tails on a glucometer chip (Accu-Check Advantage Blood Glucose Monitor, Roche Diagnostics Corp., Indianapolis, IN, USA). All db/db mice with a fasting blood glucose level >200 mg/dl were considered diabetic and included in the study (data not shown).
Alshammari M.A., Khan M.R., Majid Mahmood H., Alshehri A.O., Alasmari F.F., Alqahtani F.M., Alasmari A.F., Alsharari S.D., Alhossan A., Ahmad S.F., Nadeem A, & Alshammari T.K. (2020). Systemic TNF-α blockade attenuates anxiety and depressive-like behaviors in db/db mice through downregulation of inflammatory signaling in peripheral immune cells. Saudi Pharmaceutical Journal : SPJ, 28(5), 621-629.
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4

Oral Glucose Tolerance Test in Mice

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The animals were fasted overnight for 12 h (8 pm–8 am) with free access to water. Glucose was measured using an Accu‐Check Advantage Blood Glucose Monitor (Roche Diagnostic Corporation). Animals were fasted for 12 h, given a gavage glucose load (1.4 g/kg), and blood samples were taken at baseline and 15, 30, 60, 90, and 120 min from a cut made at the tip of the tail (Song et al., 2017 (link)).
Nascimento‐Carvalho B., Dos‐Santos A., Da Costa‐Santos N., Carvalho S.L., de Moraes O.A., Santos C.P., De Angelis K., Caperuto E.C., Irigoyen M., Scapini K.B, & Sanches I.C. (2023). Food readjustment plus exercise training improves cardiovascular autonomic control and baroreflex sensitivity in high‐fat diet‐fed ovariectomized mice. Physiological Reports, 11(5), e15609.
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5

Metabolic Profiling in Murine Diabetes

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All mice were weighed once weekly. Urine was obtained at baseline, weeks 9–10, and 13–14 in all groups using a metabolic cage. Albuminuria levels were measured by an Albumin Mouse ELISA Kit (Abcam, ab207620, Cambridge, MA, USA) and were standardized to urinary creatinine, assessed by a colorimetric assay Creatinine K® 96–300 (Labtest, Vista Alegre, Brazil) using a biochemical analyzer Cobas Mira Plus (Roche, Basel, Switzerland). The results were reported as urinary albumin-to-creatinine ratio (UACR; g/mg). Blood glucose was quantified with a glucometer (Accu-Check Advantage Blood Glucose Monitor® (Roche Diagnostic Corporation, Indianapolis, IN, USA) after six hours of fasting. All procedures were performed on animals at the beginning (9–11 weeks-old) and at the end of the protocol (14–15 weeks-old). Glycosuria was measured by the Glucose Liquiform® 133-2/500 LabTest kit (Lagoa Santa, MG, Brazil), and the absorbance values were obtained by the ChemWell-T spectrophotometer LabTest (Lagoa Santa, MG, Brazil). Homeostasis Model Assessment-Insulin Resistance (HOMA-IR) and HOMA-β, as previously described [67 (link)].
Bastos R.M., Simplício-Filho A., Sávio-Silva C., Oliveira L.F., Cruz G.N., Sousa E.H., Noronha I.L., Mangueira C.L., Quaglierini-Ribeiro H., Josefi-Rocha G.R, & Rangel É.B. (2022). Fecal Microbiota Transplant in a Pre-Clinical Model of Type 2 Diabetes Mellitus, Obesity and Diabetic Kidney Disease. International Journal of Molecular Sciences, 23(7), 3842.
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6

Intraperitoneal Glucose Tolerance Test

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An IGTT was performed on Day 55. After 12 h of fasting, 0.1 mL of blood was taken from the tail nick that rat tail was only cut once, such as other studies [31 (link)–33 (link)], and serum glucose was measured using an Accu-Check Advantage Blood Glucose Monitor (Roche Diagnostic Corporation, Indianapolis, IN, USA). Immediately after this baseline measurement, a dose of 50% glucose solution (1 g/kg body weight) was injected intraperitoneally as described previously [34 (link)]. The serum glucose level was measured at 0, 30, 60, 90, and 120 minutes after the glucose load.
Chou C.L., Pang C.Y., Lee T.J, & Fang T.C. (2015). Beneficial Effects of Calcitriol on Hypertension, Glucose Intolerance, Impairment of Endothelium-Dependent Vascular Relaxation, and Visceral Adiposity in Fructose-Fed Hypertensive Rats. PLoS ONE, 10(3), e0119843.
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7

Glucose Tolerance Test in Animals

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A glucose tolerance test (GTT) was performed at the end of the 30-day protocol in all study groups. The animals fasted for 6 hours and were given a single (i.p.) injection of glucose (1.5 g/kg). Blood samples were collected from a small cut made in the tail of each animal at 0 (zero), 5, 15, 30, 60 and 90 min after glucose load, and the area under the curve (AUC) was calculated. Blood glucose concentrations were determined using an Accu-Check Advantage Blood Glucose Monitor (Roche Diagnostic Corporation, Indianapolis, IN).
de Moraes O.A., Flues K., Scapini K.B., Mostarda C., Evangelista F.D., Rodrigues B., Dartora D.R., Fiorino P., Angelis K.D, & Irigoyen M.C. (2018). ACE gene dosage determines additional autonomic dysfunction and increases renal angiotensin II levels in diabetic mice. Clinics, 73, e246.
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