Xl dleu lamp 12cen and xl atm tp53 multi color probe kits

Peripheral blood was collected from 19 patients with CLL according to standard clinical criteria and the International Workshop on CLL (IWCLL) criteria [16 (link)] including lymphocyte morphology, Binet stage and IGHV mutational status. Deletions of 17p13, 11q22, 13q14 and trisomy 12 were detected using fluorescence in situ hybridization (FISH) with the Metasystems XL DLEU/LAMP/12cen and XL ATM/TP53 Multi-Color Probe Kits (MetaSystems, Compiègne, France). The biological and clinical characteristics of CLL patients are listed in Table 1. Peripheral blood mononuclear cells (PBMCs) were isolated from blood using Ficoll-Hypaque density gradient (1.077 g/mL) centrifugation. More than 90% of CLL PBMCs were CD19⁺CD5⁺. Freshly isolated cells were used immediately in culture assays. Cell pellets were frozen at −80 °C until RNA or protein extraction, and analysis.

Peripheral blood was collected from 52 patients (during the period from 2016 to 2018) diagnosed with CLL. Patients were diagnosed according to the International Workshop on CLL (IWCLL) criteria, and staged according to the Binet system [19 (link)]. The main exclusion criteria included neoplastic disease other than CLL, diabetes, hepatic fibrosis, and renal or lung disease. Patients were selected based on the following criteria: lymphocytosis, lymphocyte morphology, clinical features, CD5⁺/CD19⁺ immunophenotype, and IGHV mutation status. Progressive CLL was characterized by expansion of the neoplastic clone and extravascular accumulation in lymphoid tissues, the bone marrow, and other organs, giving rise to immune dysfunction, lymphadenopathy, splenomegaly and hematopoietic failure. Clinical remission is when the bone marrow contains fewer than 5% blast cells, the blood cell counts return to within normal limits, and there are no signs or symptoms of the disease. Deletions of 17p13, 11q22, 13q14 and trisomy 12 were detected using fluorescence in situ hybridization (FISH) with the Metasystems XL DLEU/LAMP/12cen and XL ATM/TP53 Multi-Color Probe Kits (MetaSystems, Compiègne, France). The patients’ clinical characteristics are summarized in Table 1.