DP cells (2 × 104 cells) were suspended in 0.5 mL EM1 medium supplemented with 0, 0.1, 1, or 10 μM OXT (Peptide Institute Inc., Japan) and seeded into the wells of a 24-well cell culture plate (Corning Inc., Corning, NY, USA). The culture medium was replaced with a fresh medium every 3 days. The cells were counted using a cell counter (Chemometec, Denmark) after 3 min of trypsin–EDTA treatment. Gene expression in DP cells was assessed using real-time reverse transcription-polymerase chain reaction (RT-PCR) after 6 days of culture.
Cell counter
Manufactured by ChemoMetec
Sourced in Denmark
The cell counter is a lab equipment designed to accurately measure and count cells. It provides precise quantification of cell samples.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using cell counter
1
Oxytocin Modulation of DP Cell Growth
2
Cinnamic Acid Effects on DP Cell Culture
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DP cells (2 × 104 cells) were suspended in 0.5 mL DPCGM medium supplemented with 0, 50, 100, 500, 1000, or 2000 μg/mL Cinnamic acid (WAKO, Japan), and seeded into the wells of a 24-well cell culture plate (Corning Inc., Corning, NY, USA). Cells were counted using a cell counter (Chemometec, Denmark) after 3 min of trypsin–EDTA treatment. Gene expression in DP cells was assessed using real-time reverse transcription-polymerase chain reaction (RT-PCR) after 3 days of culture.
3
DP Cell Culture and Gene Expression
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DP cells (2 × 104 cells) were suspended in 0.5 mL DPCGM medium supplemented with 100 μg/mL Cinnamic acid and 10 μM L-371,257 (MedChemExpress, NJ, USA), and seeded into the wells of a 24-well cell culture plate. Cells were counted using a cell counter (Chemometec, Denmark) after 3 min of trypsin–EDTA treatment. Gene expression in DP cells was assessed by RT-PCR after 3 days of culture.
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