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Ciap1

Manufactured by Merck Group
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Sourced in Australia

CIAP1 is a lab equipment product manufactured by Merck Group. It is a recombinant protein that functions as a calf intestinal alkaline phosphatase (CIAP) enzyme, which is commonly used in molecular biology applications to remove 5' phosphate groups from DNA and RNA.

Automatically generated - may contain errors

Lab products found in correlation

Ab196436, by Abcam (1 mentions) Polyvinylidene fluoride (pvdf), by Merck Group (1 mentions) Sds polyacrylamide gel, by Bio-Rad (1 mentions) Adi spa 835, by Enzo Life Sciences (1 mentions) P stat5, by Merck Group (1 mentions) α sma, by Santa Cruz Biotechnology (1 mentions)

2 protocols using ciap1

1

Western Blot Analysis of Signaling Pathways

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Cells lysates were separated on 4 to 2% gradient SDS–polyacrylamide gels (Bio-Rad), transferred to polyvinylidene fluoride (Millipore) membranes, and blotted with indicated antibodies purchased from CST except for caspase-8 (M058-3, MBL Life Science), RIPK3 (33/16-8G7-1-1, WEHI antibody facility, Australia), phospho-RIPK3 (a gift from Genentech), phospho-MLKL (ab196436, Abcam), FADD (ADI-AAM-212-E; Enzo Life Sciences), tankyrases (sc-365897, Santa Cruz Biotechnology), TRADD (sc-7868, Santa Cruz Biotechnology), PAR (MABC547; Sigma-Aldrich), RNF146 (73-233, NeuroMab), cIAP1 (clone 1E1-1-12, in house), actin (A1978, Sigma-Aldrich), and HSP90 (ADI-SPA-835, Enzo Life Sciences).
Liu L., Sandow J.J., Leslie Pedrioli D.M., Samson A.L., Silke N., Kratina T., Ambrose R.L., Doerflinger M., Hu Z., Morrish E., Chau D., Kueh A.J., Fitzibbon C., Pellegrini M., Pearson J.S., Hottiger M.O., Webb A.I., Lalaoui N, & Silke J. (2022). Tankyrase-mediated ADP-ribosylation is a regulator of TNF-induced death. Science Advances, 8(19), eabh2332.
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2

Molecular Signaling Pathway Analysis

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Human recombinant GH was obtained from the NIDDK National Hormone and Peptide Program. Mouse monoclonal Hnf6; rabbit polyclonal antibodies against mouse procaspase-3, -8, -9 and cleaved caspases -3, -8, -9, α-Sma, and Igf1 were from Santa Cruz Biotechnology, CA; mouse anti-b-actin, rabbit anti-phospho-Stat5 A/B antibodies were from Sigma-Aldrich; Ciap1, pStat5, rabbit anti-mouse horseradish peroxidase, and goat anti-mouse horseradish peroxidase were from Cell Signaling Technology.
Wang K., Wang M., Gannon M, & Holterman A. (2016). Growth Hormone Mediates Its Protective Effect in Hepatic Apoptosis through Hnf6. PLoS ONE, 11(12), e0167085.
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