Anti alpha tubulin

Manufactured by Bio-Rad

Anti-alpha tubulin is a primary antibody used to detect and visualize alpha-tubulin, a key component of the cytoskeleton in cells. It can be used in various cell biology techniques, such as Western blotting, immunocytochemistry, and immunohistochemistry, to study the distribution and dynamics of the cytoskeleton.

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Lab products found in correlation

Pvdf membrane, by Bio-Rad (3 mentions) Rabbit anti rat igg h l, by Abcam (2 mentions) Clarity max western ecl blotting substrate, by Bio-Rad (2 mentions) Goat anti rabbit igg h l hrp, by Abcam (2 mentions) Precast polyacrylamide gel, by Thermo Fisher Scientific (1 mentions) Goat anti rat hrp, by Abcam (1 mentions) Goat anti mouse hrp, by Abcam (1 mentions) Anti flag m2, by Merck Group (1 mentions) Trans blot turbo transfer system, by Bio-Rad (1 mentions) Anti rabbit igg hrp, by GE Healthcare (1 mentions) Anti β tubulin, by Merck Group (1 mentions) Anti rabbit igg starbright blue 700, by Bio-Rad (1 mentions) Anti mouse igg hrp, by Santa Cruz Biotechnology (1 mentions)

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Anti-tubulin (11 citations)

4 protocols using anti alpha tubulin

Western Blot Analysis of C. elegans

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Cell lysate were prepared from synchronized population of L4 larvae and gravid adults. 50 µg lysate were loaded onto the precast polyacrylamide gel (Thermo Fisher Scientific), subjected to electrophoresis, and transferred onto the PVDF membrane (Bio-Rad) with Trans-Blot Turbo Transfer System (Bio-Rad). The primary antibodies used were anti-FLAG (M2, Sigma) and anti-alpha-Tubulin (Bio-Rad). The secondary antibodies were goat-anti-mouse HRP (Abcam) and goat-anti-rat HRP (Abcam) respectively.

Tang W., Seth M., Tu S., Shen E.Z., Li Q., Shirayama M., Weng Z, & Mello C.C. (2018). A sex chromosome piRNA promotes robust dosage compensation and sex determination in C. elegans. Developmental cell, 44(6), 762-770.e3.

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PRG-1 Expression in C. elegans

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40 synchronized wild-type, henn-1, parn-1, and henn-1/parn-1 L1s were grown at 20°C and harvested before egg-laying for young adult protein lysate preparation. 850 embryos per strain were isolated from gravid adults grown at 20°C for embryo protein lysate preparation. Samples were separated on precast denaturing polyacrylamide gels, transfer onto PVDF membrane (Bio-Rad), and probed with anti-PRG-1 antibody (Batista et al., 2008 (link)) and rat polyclonal anti-alpha tubulin (Bio-Rad, MCA77G). HRP goat anti-rabbit IgG H&L (Abcam, ab6734) or rabbit anti-rat IgG H&L (Abcam, ab6721) secondary antibodies were used for detection using Clarity Max ECL Western Blotting Substrate (Bio-Rad).

Pastore B., Hertz H.L., Price I.F, & Tang W. (2021). pre-piRNA trimming and 2′-O-methylation protect piRNAs from 3′ tailing and degradation in C. elegans. Cell reports, 36(9), 109640.

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Protein Detection Using Commercial Antibodies

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For protein detection, the following commercially available antibodies were used. Primary antibodies: anti-OsTIR1 (MBL, #PD048), anti-mAID (MBL, #M214-3), anti-DHC1 (SantaCruz, #sc-9115), anti-SMC2 (Bethyl, #A300-058A-T), anti-CTCF (Bethyl, #A300-543-T), anti-POLR2A (Abcom, #ab817), anti-BRD4 (GeneTex, #GTX130586), anti-TOP2A (MBL, #M042-3S), anti-alpha-tubulin (MBL, #M175-3), anti-beta-tubulin (SIGMA-Aldrich, #T4026), anti-alpha-tubulin conjugated with rhodamine (Bio-Rad, 12004165). All primary antibodies were used at a 1 in 1000 dilution with TBST containing 5% skim milk. Secondary antibodies: anti-rabbit IgG HRP (GE Healthcare, #NA934), anti-mouse IgG HRP (SantaCruz, #PI-2000), anti-rabbit IgG StarBright Blue 700 (Bio-Rad, #12004161). All secondary antibodies were used at a 1 in 5000 dilution with TBST containing 5% skim milk.

Yesbolatova A., Saito Y., Kitamoto N., Makino-Itou H., Ajima R., Nakano R., Nakaoka H., Fukui K., Gamo K., Tominari Y., Takeuchi H., Saga Y., Hayashi K.I, & Kanemaki M.T. (2020). The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nature Communications, 11, 5701.

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PRG-1 Expression in C. elegans

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Pastore B., Hertz H.L., Price I.F, & Tang W. (2021). pre-piRNA trimming and 2′-O-methylation protect piRNAs from 3′ tailing and degradation in C. elegans. Cell reports, 36(9), 109640.

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