The hearts of male C57/B6 mice were dissected, immersed immediately in fresh 10% neutral buffered formalin, fixed for 16 hours at room temperature, rinsed briefly, dehydrated and embedded in paraffin. Heart were cut into 6 μm transverse sections. The TYPE-1 ViewRNA probe against Chaer LncRNA was designed by Affymetrix and was detected by ViewRNA™ ISH Tissue Assay Kit (Affymetrix, QVT0012) according to manufacturer's instructions. Briefly, tissue sections were pretreated in 90-95 °C pretreatment solution for 10 minutes, followed by protease incubation at 40°C for 35 minutes. Hybridization was performed at 40°C for 2-4 hours. After the preamplifier, amplifier and label probe 1-AP hybridization steps, probes were visualized by fast red substrate at 40°C for 40 minutes. Immunofluorescence assays were performed subsequently to detect the cardiac myocytes, endothelial cells and fibroblasts by antibodies against Troponin T Type 2 (Tnnt2; Bioworld Technology, BS6013, 1:100), isolectin b4 (ib4; Enzo Life Sciences, ALX-650-001B-MC05, 1:100) and type I collagen (MD Biosciences, 203002, 1:200), respectively. Images were taken by Olympus FluoView™ FV1000 confocal laser scanning microscope Brochure.
Fluoview fv1000 confocal laser scanning microscope brochure
Manufactured by Olympus
The FluoView™ FV1000 is a confocal laser scanning microscope designed for high-resolution imaging. It utilizes a laser-based scanning system to capture detailed images of samples. The FV1000 is capable of producing high-quality, high-contrast images by selectively illuminating and detecting fluorescent signals within the sample.
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2 protocols using fluoview fv1000 confocal laser scanning microscope brochure
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Probing Cardiac Chaer lncRNA Expression
2
Probing Cardiac Chaer lncRNA Expression
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The hearts of male C57/B6 mice were dissected, immersed immediately in fresh 10% neutral buffered formalin, fixed for 16 hours at room temperature, rinsed briefly, dehydrated and embedded in paraffin. Heart were cut into 6 μm transverse sections. The TYPE-1 ViewRNA probe against Chaer LncRNA was designed by Affymetrix and was detected by ViewRNA™ ISH Tissue Assay Kit (Affymetrix, QVT0012) according to manufacturer's instructions. Briefly, tissue sections were pretreated in 90-95 °C pretreatment solution for 10 minutes, followed by protease incubation at 40°C for 35 minutes. Hybridization was performed at 40°C for 2-4 hours. After the preamplifier, amplifier and label probe 1-AP hybridization steps, probes were visualized by fast red substrate at 40°C for 40 minutes. Immunofluorescence assays were performed subsequently to detect the cardiac myocytes, endothelial cells and fibroblasts by antibodies against Troponin T Type 2 (Tnnt2; Bioworld Technology, BS6013, 1:100), isolectin b4 (ib4; Enzo Life Sciences, ALX-650-001B-MC05, 1:100) and type I collagen (MD Biosciences, 203002, 1:200), respectively. Images were taken by Olympus FluoView™ FV1000 confocal laser scanning microscope Brochure.
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