In the current study, chemicals of extra-pure analytical grade and organic solvents of HPLC grade were used. NZ pure standard (assay (HPLC) = 98.0%), lot No. ALR6837, was from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Acetonitrile (HPLC grade) was obtained from Kanto Chemical Co., Inc. (Tokyo, Japan). Hydrochloric acid (35–37%), sodium carbonate, and sodium nitrite were obtained from Nacalai Tesque, Inc. (Kyoto, Japan). Ammonium acetate, sodium chloride, and pepsin from porcine gastric mucosa (powder ≥250 units/mg solid) were products of Sigma–Aldrich (St. Louis, MO, USA). SGJ (pH 1.2) was prepared as per the United States Pharmacopoeia (USP) [9 ] by dissolving 2 g of sodium chloride and 3.2 g of pepsin in 7 mL HCl and sufficient water was added to make 1000 mL.
Sodium nitrite
Manufactured by Nacalai Tesque
Sourced in Japan
Sodium nitrite is a chemical compound that is commonly used in various laboratory applications. It is a white or slightly yellowish crystalline solid with the chemical formula NaNO2. Sodium nitrite serves as an oxidizing agent and is often utilized in analytical chemistry, biochemistry, and materials science experiments.
Automatically generated - may contain errors
Lab products found in correlation
Sodium nitrate, by Nacalai Tesque (3 mentions)
Sodium iodate, by Nacalai Tesque (2 mentions)
Ammonium chloride, by Nacalai Tesque (2 mentions)
Sodium bromate, by Nacalai Tesque (2 mentions)
Sodium iodide, by Nacalai Tesque (2 mentions)
Sodium thiocyanate, by Nacalai Tesque (2 mentions)
Sodium bromide, by Nacalai Tesque (2 mentions)
Simplicity uv water purification system, by Merck Group (2 mentions)
Porcine gastric mucosa, by Merck Group (1 mentions)
Acetonitrile hplc grade, by Kanto Chemical (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Pepsin, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
3 trimethoxysilylpropyl methacrylate, by Tokyo Chemical Industry (1 mentions)
Fused silica capillary tube, by GL Sciences (1 mentions)
2 methacryloyloxy ethyl dimethyl 3 sulfopropyl ammoniumhydroxide, by Merck Group (1 mentions)
Tskgel nh2 60, by Tosoh (1 mentions)
Zic hilic, by Merck Group (1 mentions)
Tskgel amide 80, by Tosoh (1 mentions)
4 protocols using sodium nitrite
1
Preparation and Characterization of Simulated Gastric Juice
2
Reagent Preparation for Analytical Research
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All reagents were guaranteed-grade products and obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan), unless noted otherwise. 3-(Trimethoxysilyl) propyl methacryrate was obtained from Tokyo Chemical Industry Co., Ltd., (Tokyo, Japan), and 2AM18C6E was obtained from Sigma-Aldrich Co. LLC (St. Louis, USA). Sodium iodate, sodium bromate, sodium nitrate, sodium nitrite, ammonium chloride, and cesium chloride were obtained from Nacalai Tesque, Inc., (Kyoto, Japan). Calcium chloride, rubidium chloride, and lithium chloride were obtained from Yoneyama Yakuhin Kogyo Co., Ltd., (Osaka, Japan).
3
Synthesis and Characterization of Zwitterionic Polymer Packing
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The reagents employed were of guaranteed grade reagents and were obtained from Wako Pure Chemical Industries (Osaka, Japan), unless otherwise noted. Sodium bromide, sodium iodate, sodium iodide, sodium nitrate, sodium nitrite, sodium thiocyanate, ammonium chloride and benzyltrimethylammonium chloride were obtained from Nacalai Tesque (Kyoto, Japan). 3-(Trimethoxysilyl)propyl methacrylate was obtained from Tokyo Chemical Industry (Tokyo, Japan). Lithium chloride, magnesium chloride hexahydrate and potassium chloride were obtained from Yoneyama Yakuhin Kogyo (Osaka, Japan).
[2-(Methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide (zwitterionic monomer) was obtained from Sigma-Aldrich (St. Louis, USA). Ultrapure water was prepared in the laboratory by using a Simplicity UV water purification system (Millipore, MA, USA), and all solutions used in this study were prepared using this ultrapure water. All packing materials were packed in fused-silica capillary tube (100 × 0.32 mm i.d.; GL Sciences, Tokyo, Japan).
[2-(Methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide (zwitterionic monomer) was obtained from Sigma-Aldrich (St. Louis, USA). Ultrapure water was prepared in the laboratory by using a Simplicity UV water purification system (Millipore, MA, USA), and all solutions used in this study were prepared using this ultrapure water. All packing materials were packed in fused-silica capillary tube (100 × 0.32 mm i.d.; GL Sciences, Tokyo, Japan).
4
Comparative Evaluation of HILIC Columns
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The reagents employed were of guaranteed reagent grade and were obtained from Wako Pure Chemical Industries (Osaka, Japan), unless otherwise noted. Sodium bromate, sodium bromide, sodium iodide, sodium nitrate, sodium nitrite, and sodium thiocyanate were obtained from Nacalai Tesque (Kyoto, Japan). HPLC-grade acetonitrile was obtained from Tokyo Chemical Industry (Tokyo, Japan). Ultrapure water was prepared in the laboratory by using a Simplicity UV water purification system (Millipore, Molsheim, France), and all solutions used in this study were prepared using this ultrapure water.
TSKgel NH2-60 and TSKgel Amide-80 were obtained from Tosoh Corporation (Yamaguchi, Japan). Polar-Imidazole and Polar-Pyridine were obtained from Sepax Technologies (Newark, USA), while ZIC-HILIC was obtained from Merck Millipore (Darmstadt, Germany). The specifications of the employed columns are summarized in Table 1. All packing materials were packed in a fused-silica capillary tube (100 × 0.32 mm i.d.).
TSKgel NH2-60 and TSKgel Amide-80 were obtained from Tosoh Corporation (Yamaguchi, Japan). Polar-Imidazole and Polar-Pyridine were obtained from Sepax Technologies (Newark, USA), while ZIC-HILIC was obtained from Merck Millipore (Darmstadt, Germany). The specifications of the employed columns are summarized in Table 1. All packing materials were packed in a fused-silica capillary tube (100 × 0.32 mm i.d.).
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