To document structural changes that occur following acid treatment, eight seeds each treated with HCl for 15, 30, 45 or 90 min were mounted on aluminum SEM stubs and coated with gold alloy using a Technics Hummer VI sputter coater. These stubs containing seeds were then scanned in a FEI Quanta 450 field emission scanning electron microscope, and micrographs were compared with the impermeable seeds, that is seeds that had been dried for 60 hours and did not absorb water during imbibition test.
Quanta 450 field emission scanning electron microscope
Manufactured by Thermo Fisher Scientific
Sourced in Austria
The Quanta 450 is a field emission scanning electron microscope (FESEM) designed for high-resolution imaging and analysis of a wide range of samples. It provides excellent resolution, low-voltage imaging, and a variety of analytical capabilities.
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Lab products found in correlation
3 protocols using quanta 450 field emission scanning electron microscope
1
Acid-Induced Structural Changes in Seeds
2
Characterizing Optimized Lipid Carrier Morphology
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The optimized formulations LC5 and LC8 were chosen to represent each group’s morphology due to the formulation’s stability, the concentration of HA, and the variability in parameters such as particle size, zeta potential, %EE, and %drug release.
Surface morphology and composition of LC samples were analyzed by scanning electron microscopy (SEM, FEI Quanta 450 Field Emission Scanning Electron Microscope, Austria). LC samples were pretreated by four-fold dilution with DI water sonicated for 5 mins, then mounted on a stub, gold-coated, and observed at 30,000× magnification.
The LC samples’ morphological characteristics were observed by transmission electron microscopy (TEM, FEI Company, USA). TEM was observed at 10,000–20,000× magnification after a drop of each four-fold diluted sample was applied to a copper grid coated with carbon film and air-dried.
Surface morphology and composition of LC samples were analyzed by scanning electron microscopy (SEM, FEI Quanta 450 Field Emission Scanning Electron Microscope, Austria). LC samples were pretreated by four-fold dilution with DI water sonicated for 5 mins, then mounted on a stub, gold-coated, and observed at 30,000× magnification.
The LC samples’ morphological characteristics were observed by transmission electron microscopy (TEM, FEI Company, USA). TEM was observed at 10,000–20,000× magnification after a drop of each four-fold diluted sample was applied to a copper grid coated with carbon film and air-dried.
3
Cryogenic SEM Imaging of Composite Gel
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An FEI Quanta
450 field emission scanning electron microscope was used to observe
the morphology of the composite gel. When using the scanning electron
microscope to observe the gel aggregates, no coating was required.
The sample preparation temperature was −196 °C.
450 field emission scanning electron microscope was used to observe
the morphology of the composite gel. When using the scanning electron
microscope to observe the gel aggregates, no coating was required.
The sample preparation temperature was −196 °C.
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