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2 protocols using antibodies against phospho stat3 tyr 705

1

Ovarian Cancer Cell Lines Protocol

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Antibodies against TRIM47, β-actin, Bax, Bcl-2, E-cadherin, vimentin, and STAT3 were purchased from Proteintech (Wuhan, China). Antibodies against phospho-STAT3 (Tyr-705) were obtained from Cell Signaling Technology (Danvers, MA, USA). The secondary antibody for western blotting was purchased from Servicebio (Wuhan, China). EdU proliferation supplies, CCK-8 kit, and enhanced chemiluminescent reagent were acquired from the Boyetime (Shanghai, China). TRIzol® reagent was purchased from Invitrogen (Grand Island, NY, USA). The human ovarian cancer cell lines SKOV3 and OVCAR3 were purchased from the Chinese Academy of Sciences Cell Bank (Shanghai, China) and grown in RPMI-1640 medium (Keygen Biotech, Nanjing, China) supplemented with 10% FBS (Gibco, Grand Island, NY, USA) in a humidified environment with 5% CO2 at 37°C.
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2

Inflammatory Cytokine Quantification Protocol

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OVA (grade V) was obtained from Sigma (USA), aluminum hydroxide gel was obtained from Thermo (USA) and anti-rat IL-6/IL-1β/TNF-α enzyme-linked immunosorbent assay (ELISA) kits were obtained from Feiya Biotech (China). Baicalein was provided by Dr Qinglong Guo of the College of Pharmacy at China Pharmaceutical University. Antibodies against phospho-STAT3 (Tyr705) were obtained from Cell Signaling Technology (USA), which were used for immunohistochemical staining at 1:100 dilution. BCA Protein Assay kit was purchased from Thermo Fisher Scientific (USA). The kit for immunohistochemical staining was obtained from Keygen biotechnology Co., Ltd. (China). Diaminobenzidine (DAB) was obtained from Solarbio Science & Technology Co., Ltd. (China). Hematoxylin and eosin were purchased from Solarbio Science & Technology Co., Ltd. (China).
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