Descriptive results were expressed as means with SD. Two-way GLMRM analysis (IBM
SPSS statistics, ver. 22) was used to determine the effect, and interaction between the types of intervention (two control and three intervention groups) and the treatment (pre-post), on the variables measured from the blood. If a significant effect or interaction was detected, then post-hoc tests with Bonferroni adjustment were performed to determine where the differences existed. Statistical significance was set at the alpha level of 0.05.
The measurements of proteins expressions from skeletal muscle were only available at post-treatment. Therefore, one-way ANOVA (IBM
SPSS statistics, ver. 22) was used to determine the effects of interventions (five groups) on the expressions of GLUT4, Akt, phosphorylated IR and GSK3 and the Akt activity from skeletal muscle at post-treatment. If a significant effect was detected, then post-hoc test with Bonferroni adjustment was used to determine where the difference existed. Statistical significance was set at the alpha level of 0.05.
This research obtained approval by the Animal Care and Ethics Committee of Southern Cross University (ARA-13/04 and ARA-14/09).
Wang Y., Wen L., Zhou S., Zhang Y., Wang X.H., He Y.Y., Davie A, & Broadbent S. (2018). Effects of four weeks intermittent hypoxia intervention on glucose homeostasis, insulin sensitivity, GLUT4 translocation, insulin receptor phosphorylation, and Akt activity in skeletal muscle of obese mice with type 2 diabetes. PLoS ONE, 13(9), e0203551.