The mice were anaesthetized, and the pupils were dilated by applying Cyclomydril (Alcon, Fort Worth, TX, USA). Using a 532-nm laser, a slit-lamp delivery system, and a sliding glass as a contact lens, 4 spots (laser power, 200 mW; exposure time, 100 ms; hole size, 75 μm) were placed into each eye at a distance of approximately 2 optic disc diameters from the optic nerve head. After the laser-induced injury, the clodronate group mice (> 20 weeks old) received an intravitreal injection with 2 μl/eye of 90 μg clodronate liposomes (including 16 μg of clodronic acid) mixed with 154 mM sodium chloride at a ratio of 1:1 or control liposomes using a 33-gauge needle. The eyes of a selective cyclooxygenase-2 (COX2) inhibitor celecoxib (Cayman Chemical Company #169,590–42-5) injection group were subjected to IVI of celecoxib (10 mM celecoxib dissolved in 154 mM sodium chloride at a ratio of 1:3), or DMSO (vehicle, 25%). In some cases, celecoxib was administered orally at a dose of 100 mg/kg/day. celecoxib was dissolved in 100% ethanol (v/v) and mixed with the food. After the ethanol was completely volatilized, the food was fed to the mice daily. The control mice were fed with the volatilized ethanol-treated food daily.
Celecoxib
Manufactured by Cayman Chemical
Sourced in United States
Celecoxib is a chemical compound commonly used as a reference standard in analytical procedures. It is a selective cyclooxygenase-2 (COX-2) inhibitor. The core function of Celecoxib is to serve as a reference material for the identification and quantification of this compound in various analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Nifedipine, by Merck Group (2 mentions)
Sildenafil, by Cayman Chemical (2 mentions)
Gentamicin, by Harvard Bioscience (2 mentions)
Amphotericin, by Harvard Bioscience (2 mentions)
Ns 398, by Cayman Chemical (2 mentions)
Sc 560, by Cayman Chemical (2 mentions)
Sc 236, by Cayman Chemical (2 mentions)
Doxorubicin, by Merck Group (2 mentions)
Cay10526, by Cayman Chemical (2 mentions)
Ibuprofen, by Cayman Chemical (2 mentions)
Aspirin, by Cayman Chemical (2 mentions)
Standard mouse chow, by Inotiv (1 mentions)
Diclofenac, by Cayman Chemical (1 mentions)
Adenosine, by Merck Group (1 mentions)
Tamsulosin hydrochloride, by Merck Group (1 mentions)
Butoxamine, by Merck Group (1 mentions)
Mirabegron, by Cayman Chemical (1 mentions)
Vardenafil, by Cayman Chemical (1 mentions)
Isoproterenol, by Merck Group (1 mentions)
Y 27632 dihydrochloride, by R&D Systems (1 mentions)
27 protocols using celecoxib
1
Laser-Induced Retinal Injury Model
2
Listeria Monocytogenes Infection Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
L. monocytogenes of the wild-type, attenuated (LADD), or Δhly background were grown overnight in brain heart infusion media at 30C. The bacteria were back diluted 1:5 and allowed to grow to log phase (OD0.4–0.6, ~1–1.5 hours) at 37C, with aeration, prior to infection. Bacteria were diluted in PBS and mice were infected with 200μL at the indicated doses intravenously. For bacterial burden analysis, mice were sacrificed at 12hpi and livers were homogenized in 0.1% Nonidet P-40 in PBS and plated on Luria-Bertani plates. For splenic macrophage depletion, 200μL clodronate, PBS control, or endosome lipid control (Encapsula Nano Sciences) were given intravenously 24 hours prior to bacterial infection according to the manufacturer’s instructions. For B-cell depletion, mice were dosed intraperitoneally with 12.5μg of Ultra-LEAF Purified anti-mouse CD20 (clone SA271G2) or isotype control (clone RTK4530) in 100μL PBS 24 hours prior to bacterial infection. Depletion efficacy of relevant cell subsets was confirmed by assessing abundance of splenic CD11b+ cells (clone M1/70), CD11c+ cells (clone N418), or B220+ cells (clone RA3-6B2) by flow cytometry. Celecoxib (Cayman Chemical) was milled into standard mouse chow (Envigo) at 100mg/kg and fed ad lib for 48 hours before and after immunization [16 (link),77 (link)].
3
Synthesis and Characterization of Compound I
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Compound I (2-methyl-2-[cis-4-([1-(6-methyl-3-phenylquinolin-2-yl)piperidin-4-yl]carbonyl amino)cyclohexyl] propanoic acid) was synthesized at Medicinal Chemistry Research Laboratories, Daiichi Sankyo Co., Ltd. Detailed information of its chemical synthesis is described in the patent [18 ]. The chemical structure is shown in Figure 1 . IP antagonist, RO3244019, (R)-3-phenyl-2-(5-phenyl-benzofuran-2-ylmethoxycarbonylamino)-propionic acid, and EP4 antagonist, CJ-023423, N-[N-[2-[4-(2-ethyl-4,6-dimethyl-1H-imidazo[4,5-c]pyridin-1-yl)phenyl]ethyl]carbamoyl]-4-methylbenzenesulfonamide, were synthesized at Medicinal Chemistry Research Laboratories, Daiichi Sankyo Co., Ltd. Celecoxib (4-[5-(4-methylphenyl)-3-(trifluoromethyl)pyrazol-1-yl]benzenesulfonamide) was purchased from Cayman Chemical Company (Ann Arbor, MI). All compounds were suspended in 0.5% methyl cellulose 400 solution and sterilized (Wako Pure Chemical Industries, Ltd., Osaka, Japan) and a volume of 5 mL/kg was administered orally to animals.
4
Preparation of Compound Stock Solutions
5
Melanoma cell lines BRAF-V600E assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Two human melanoma cell lines were used: A375 (European Collection of Authenticated Cell Cultures, through Sigma Aldrich Company, St. Louis, MO, USA) and SK-MEL-28 (Cell line services, Eppelheim, Germany). Both melanoma cell lines were positive for BRAF-V600E mutation. Each cell line was seeded and maintained in Dulbecco’s modified Eagle Medium (DMEM), enriched with 10% fetal calf serum (FCS), 5 ng/mL amphotericin, and 50 μg/mL gentamicin, all reagents being from Biochrom AG, Berlin, Germany. The protocol followed standard culture conditions: cells were kept at constant temperature (37 °C) and humidity, under a 5% CO2 atmosphere, in an incubator, and were fed twice a week; all experiments were carried out in triplicate (n = 3); cells were used within a maximum of 4 passages. Dabrafenib and celecoxib were purchased from Cayman Chemical, MI, USA.
6
Amnion Biomechanics Under Inflammatory Conditions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Then 3- ×3-cm sections of amnion from CS-term pregnancies were prepared from
the region measuring 5 cm away from the edge of the placental disk and were incubated
in media (no drug control), 10 ng/mL TNF-α (Peprotech), 0.5%
ethacridine (Abcam), 10−5 M Etodolac (Sigma Aldrich, St. Louis,
MO), 10 μM celecoxib (Cayman, Ann Arbor, MI), and 10 ng/mL
TNF-α with 10−5 M Etodolac at
37°C overnight. The treated amnion was placed to cover the end of a
pressurized tube 1 cm in diameter and placed within a DMEM bath. The tube was
connected to a syringe pump, which produced pressurized flow of infused DMEM.
Membrane rupture was signaled by blue dye from the DMEM entering the DMEM bath.
Pressure and time of rupture was recorded. Western blot was performed on all ruptured
membranes.
the region measuring 5 cm away from the edge of the placental disk and were incubated
in media (no drug control), 10 ng/mL TNF-α (Peprotech), 0.5%
ethacridine (Abcam), 10−5 M Etodolac (Sigma Aldrich, St. Louis,
MO), 10 μM celecoxib (Cayman, Ann Arbor, MI), and 10 ng/mL
TNF-α with 10−5 M Etodolac at
37°C overnight. The treated amnion was placed to cover the end of a
pressurized tube 1 cm in diameter and placed within a DMEM bath. The tube was
connected to a syringe pump, which produced pressurized flow of infused DMEM.
Membrane rupture was signaled by blue dye from the DMEM entering the DMEM bath.
Pressure and time of rupture was recorded. Western blot was performed on all ruptured
membranes.
7
Celecoxib and Prostaglandin Modulation in Infection
8
Porcine Sapovirus Capsid and VPg Antibody Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Celecoxib, NS-398, SC-58125, SC-236, nimesulide, and SC-560 were purchased from Cayman Chemical (Ann Arbor, MI, USA). GCDCA, dimethyl sulfoxide (DMSO), L-NAME, MDL-12330A, and indomethacin were from Sigma-Aldrich (St. Louis, MO, USA). COX-1 siRNA, COX-2 siRNA, and scrambled siRNA were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Monoclonal antibody (MAb) against mouse COX-1 and polyclonal antibody against rabbit COX-2 were obtained from Abcam (Cambridge, MA, USA). Mouse MAb against HA tag was purchased from OriGene (Rockville, MD, USA). Synthetic PGE2 was purchased from Tocris Bioscience (Ellisville, MO, USA). The anti-PSaV capsid MAb and the anti-PSaV VPg polyclonal antibody were previously described (41 (link)). The secondary antibodies used were horseradish peroxidase-conjugated goat immunoglobulin against rabbit IgG (Cell Signaling, Beverly, MA, USA) and mouse IgG (Santa Cruz) and fluorescein isothiocyanate (FITC)-conjugated goat immunoglobulin against rabbit IgG (Jackson Immuno Research Laboratory, West Grove, PA, USA).
9
Cetuximab and COX-2 Inhibitors Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cetuximab (Erbitux) was obtained from the outpatient pharmacy at The University of Texas MD Anderson Cancer Center. The COX-2 inhibitors celecoxib, SC-236 (4-[5-(4-chlorophenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]-benzenesulfonamide), and NS-398 (9N-[2-(cyclohexyloxy)-4-nitrophenyl]-methanesulfonamide) were purchased from Cayman Chemical. All other chemicals were purchased from Sigma-Aldrich unless otherwise specified.
10
Zebrafish Daily Drug Injections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Zebrafish were injected daily, beginning on the day of surgery, with 220 ng/g of NS-398 or 1 μg/g Celecoxib (Cayman Chemical, Ann Arbor, MI #70590, #10008672); vehicle controls were 0.1% DMSO, or 0.44% EtOH, respectively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!