Twenty seven established cutaneous melanoma cell lines were used in this study. Twenty three early-passage (<20) melanoma lines were established from AJCC stage III and IV melanoma patients who received elective surgery at JWCI and were authenticated with autologous peripheral blood leukocytes. Stage I/II melanoma lines (WC00060, WC00080, WC00081 and WC00062) were obtained from Coriell Institute (Camden, NJ) and have been tested by short tandem repeat DNA profiles. Cells were cultured in a humidified chamber with 5% CO2 at 37°C in either RPMI-1640 (Corning, Corning, NY) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gemini Bio-Products, Sacramento, CA) and 1% penicillin-streptomycin, or MCDB-153 (Sigma-Aldrich, St. Louis, MO) / Leibovitz’s L-15 (Life Technologies, Carlsbad, CA) supplemented with 2% FBS, Insulin (5 μg/ml, Sigma-Aldrich), and 1% penicillin-streptomycin (9 (link),17 (link)). All the cell line experiments were completed within 12 passages or <4 months from thawing.
Wc00062
Manufactured by Coriell Institute for Medical Research
Sourced in United States
The WC00062 is a laboratory equipment designed for liquid sample handling and dispensing. It is a precision instrument used for accurately transferring small volumes of liquids. The core function of this product is to facilitate efficient and reliable liquid manipulation in various scientific applications.
Automatically generated - may contain errors
Lab products found in correlation
Wc00060, by Coriell Institute for Medical Research (2 mentions)
Wc00081, by Coriell Institute for Medical Research (2 mentions)
Wc00080, by Coriell Institute for Medical Research (1 mentions)
Mcdb153, by Merck Group (1 mentions)
Leibovitz s l 15, by Thermo Fisher Scientific (1 mentions)
Insulin, by Merck Group (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Gemini Bio (1 mentions)
Rpmi 1640, by Corning (1 mentions)
2 protocols using wc00062
1
Melanoma Cell Line Characterization
2
Cell Line Authentication and Genetic Modification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The WM-115 and WM-266-4 cell lines were purchased from the European Collection of Authenticated Cell Culture (ECACC), were subjected to cell line authentication via short tandem repeat profiling and were grown up to a maximum of 20 passages and for fewer than 6 months following resuscitation in our laboratory. The WM-266-4 GFP cell line was obtained by transduction with WPXLd, a lentiviral vector encoding GFP, and selected by flow cytometry. Additionally, four primary melanoma cell lines (WC00060, WC00062, WC0008 and WC00081) were purchased from the Coriell Cell Repository (Coriell Institute for Medical Research, Camden, NJ, USA).
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