For fatty acid composition analysis, 150 mL of a solvent (CM) in which chloroform and methanol were mixed at a ratio of 2:1 was added to 5 g of the sample, homogenized at 2500× g for 3 min, filtered, and 20 mL of 0.88% KCl was added. Then, sodium sulfate was added to the resultant lower layer after centrifugation at 3000 rpm for 10 min and filtered. The sample was concentrated between 560–565 °C using a rotary evaporator (N-1000, Eyela, Tokyo, Japan), the concentrated lipids were sealed with para film after injection of nitrogen gas, and stored frozen at −20 °C until methylation. To 200 μL of the sample, 1 mL of 0.5 N NaOH and 2 mL of 14% boron trifluoride were added, heated at 80 °C for 1 h and cooled for 10 min. Then, 5 mL of the sample was mixed with 2 mL of heptane, 2 mL of saturated NaCl solution was added, and left for 30 min. Then, 100 μL of the supernatant was taken and analyzed by gas chromatography (Agilent 7890, Agilent, Santa Clara, CA, USA). The column of the GC was HP-INNOWAX (30 m × 0.25 nm ID, 0.25 μm film) (Agilent 7890, Agilent, Santa Clara, CA, USA); the detector temperature was 260 °C; the injector temperature was 260 °C; the oven temperature was 100 °C for 2 min, 3 °C for a min, and 230 °C for 20 min; and nitrogen was used as the carrier gas.
Agilent 7890
Manufactured by Agilent Technologies
Sourced in United States, Canada
The Agilent 7890 is a gas chromatograph designed for the separation and analysis of a wide range of chemical compounds. It features a precise temperature-controlled oven, advanced detectors, and intuitive software to facilitate accurate and efficient sample analysis.
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Lab products found in correlation
Db 5ms capillary column, by Agilent Technologies (6 mentions)
Agilent 5975c, by Agilent Technologies (6 mentions)
Gc ead pro version 4, by Syntech (3 mentions)
Hp 5ms capillary column, by Agilent Technologies (3 mentions)
Agilent 5975, by Agilent Technologies (3 mentions)
Hp innowax column, by Agilent Technologies (3 mentions)
Pegasus 4d time of flight mass spectrometer, by Leco (2 mentions)
Electronic tissue disruptor, by Qiagen (2 mentions)
Agilent 5973n, by Agilent Technologies (2 mentions)
Speedvac, by Thermo Fisher Scientific (2 mentions)
5975c mass selective detector, by Agilent Technologies (2 mentions)
Agilent 6890, by Agilent Technologies (2 mentions)
Hp 5ms column, by Agilent Technologies (2 mentions)
Prg 2 eag 10x gain probe, by Syntech (2 mentions)
Db 5ms, by Agilent Technologies (2 mentions)
Agilent 7890a, by Agilent Technologies (2 mentions)
Acquity 1 class, by Waters Corporation (2 mentions)
Agilent ms model 5975c msd, by Agilent Technologies (2 mentions)
Db 1701, by Agilent Technologies (2 mentions)
Pegasus 4d, by Agilent Technologies (2 mentions)
131 protocols using agilent 7890
1
Fatty Acid Composition Analysis Protocol
2
Plastic Degradation in Supercritical CO2
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Plastic degradation efficiency (Y) was calculated using Eq. 1 . Products obtained from the degradation of PS in Sc-CO2 included gases, liquids and solid residues, and these products were analyzed using different methods. The components of gases products were analyzed using gas chromatograph (GC, Agilent 7890, Agilent Technologies, Santa Clara, CA, USA). Solid residues were analyzed by scanning electron microscope (SEM, Superscan SSX-550, Kyoto, Japan) and energy dispersive X-ray spectroscope (EDS) for their morphologies and elements. Liquid products obtained at the end of reactions were analyzed using a gas chromatograph-mass spectrometer (GC–MS, Agilent 7890, Agilent Technologies, Santa Clara, CA, USA). where m is the mass of the initial PS, i.e., 0.15 g; m0 is the mass of the solid residue after degradation.
3
Automated Extraction of Volatile Compounds from Wine
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The extraction of volatile compounds was automatically performed by using a CombiPal system (CTC Analytics AG, Zwingen, Switzerland) provided with a 50/30 µm Divinylbencene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS) fiber of 2 cm length (Supelco, Bellefonte, PA. USA). 5 ml of wine sample and 2 g NaCl were placed in 15 ml sample vial sample vial with 10 μl of internal standard (methyl nonanoate 15ppm). The vial was capped with a PTFE-silicon septum. The extraction was performed in the headspace of the vial for 20 minutes at 40 ºC.
The desorption was performed in the injector of the GC chromatograph (Agilent 7890) in splitless mode for 12 minutes at 280 ºC. After each injection the fiber was cleaning for 30 minutes avoiding any memory effect. All the analyses were performed in triplicate. An Agilent MSD ChemStation Software was used to control the gas chromatograph (Agilent 7890). For separation, a fused silica CP-WAX 57CB column (50m X 0.25mm X 0.39mm film thickness) from Varian (Houten, The Netherlands) was used. Helium was the carrier gas (1 ml/min).
The oven temperature was programmed as follows: 60 ºC as initial temperature, held for 5 minutes, followed by a ramp of temperature at 2 ºC/min to 120 ºC and
The desorption was performed in the injector of the GC chromatograph (Agilent 7890) in splitless mode for 12 minutes at 280 ºC. After each injection the fiber was cleaning for 30 minutes avoiding any memory effect. All the analyses were performed in triplicate. An Agilent MSD ChemStation Software was used to control the gas chromatograph (Agilent 7890). For separation, a fused silica CP-WAX 57CB column (50m X 0.25mm X 0.39mm film thickness) from Varian (Houten, The Netherlands) was used. Helium was the carrier gas (1 ml/min).
The oven temperature was programmed as follows: 60 ºC as initial temperature, held for 5 minutes, followed by a ramp of temperature at 2 ºC/min to 120 ºC and
4
Comprehensive GCMS Analysis of Compounds
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The gas chromatography mass spectroscopy (GCMS) was performed by Agilent 7890 coupled to JeolAccuTOF GCV, USA.
Make of GC: Agilent 7890; FID detector. Make of MS: Model -JeolAccuTOF GCV, USA; EI/CI source; time of flight analyser; Mass range: 10-2000 amu, mass resolution: 6000.
Operation conditions: Ionization mode: EI+; Ionization volt: 70 eV Column specification: length-30 m, thickness-0.25 μm, internal diameter-25 mm Helium was used as carrier gas at a flow rate of 1 mL/ min.
The temperature raised from 70 °C up to 280 °C with the rate of 8 °C per min rise in temperature.
The analysis was carried out at Sophisticated Analytical Instrument Facility, Indian Institute of Technology, Bombay. The outcomes of mass spectra were compared with those stored in spectrometer database of National Institute of Standards and Technology Mass Spectral Database (NIST-MS). The Kovats retention index of each compound was also confirmed.
The Structure Data Format and canonical SMILES of the compounds identified by GCMS were downloaded from PUBCHEM.
Make of GC: Agilent 7890; FID detector. Make of MS: Model -JeolAccuTOF GCV, USA; EI/CI source; time of flight analyser; Mass range: 10-2000 amu, mass resolution: 6000.
Operation conditions: Ionization mode: EI+; Ionization volt: 70 eV Column specification: length-30 m, thickness-0.25 μm, internal diameter-25 mm Helium was used as carrier gas at a flow rate of 1 mL/ min.
The temperature raised from 70 °C up to 280 °C with the rate of 8 °C per min rise in temperature.
The analysis was carried out at Sophisticated Analytical Instrument Facility, Indian Institute of Technology, Bombay. The outcomes of mass spectra were compared with those stored in spectrometer database of National Institute of Standards and Technology Mass Spectral Database (NIST-MS). The Kovats retention index of each compound was also confirmed.
The Structure Data Format and canonical SMILES of the compounds identified by GCMS were downloaded from PUBCHEM.
5
GC-MS/TOF Analysis of Serum and Urine
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GC-MS/TOF analysis was performed using the Agilent 7890 gas chromatograph system coupled with a Pegasus HT time-of-flight mass spectrometer. The system utilized a DB-5MS capillary column coated with 5% diphenyl cross-linked with 95% dimethylpolysiloxane (30 m × 250 μm inner diameter, 0.25 μm film thickness; J&W Scientific, Folsom, CA, USA).
1 μL (serum) and 2 μL (urine) aliquots were injected through a vaporizing injector in the splitless mode. Helium was used as the carrier gas; the front inlet purge flow was set at 3 mL min−1, and the gas flow rate through the column was set at 1 mL min−1. The initial temperature was kept at 50 °C for 1 min, then raised to 310 °C at a rate of 20 °C min−1 (serum) and 10 °C min−1 (urine), and finally kept for 6 min (serum) and 3 min (urine) at 310 °C. The injection, the transfer line, and the ion source temperatures were set at 280, 270, and 220 °C, respectively. The energy was −70 eV in electron impact mode. The mass spectrometry data were acquired in full-scan mode with the m/z range of 30–600 (serum) and 50–500 (urine) at a rate of 20 spectra per second after a solvent delay of 366 seconds (serum) and 455 seconds (urine).
1 μL (serum) and 2 μL (urine) aliquots were injected through a vaporizing injector in the splitless mode. Helium was used as the carrier gas; the front inlet purge flow was set at 3 mL min−1, and the gas flow rate through the column was set at 1 mL min−1. The initial temperature was kept at 50 °C for 1 min, then raised to 310 °C at a rate of 20 °C min−1 (serum) and 10 °C min−1 (urine), and finally kept for 6 min (serum) and 3 min (urine) at 310 °C. The injection, the transfer line, and the ion source temperatures were set at 280, 270, and 220 °C, respectively. The energy was −70 eV in electron impact mode. The mass spectrometry data were acquired in full-scan mode with the m/z range of 30–600 (serum) and 50–500 (urine) at a rate of 20 spectra per second after a solvent delay of 366 seconds (serum) and 455 seconds (urine).
6
Measuring Greenhouse Gas Emissions
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On each sampling date, gas emissions were measured with four closed chambers per site. With each chamber, the measurements were repeated four to six times per day and site, resulting in 15–24 net surface rate measurements. Excessive vegetation was removed before pressing the stainless steel chambers (A = 150 cm2, V = 1800 ml) into the soil [54 (link)]. The chambers had a sharp-edged bottom, which allowed the installation in the organic soils without compacting the soil. Gas samples (12 ml) were taken with syringes from the headspace immediately, 20, 40, and 60 min after closing the chambers via a three-way stopcock, and transferred into pre-evacuated exetainers (5.9 ml, Labco Lt, UK). Gas concentrations were measured on an Agilent 7890 gas chromatograph equipped with a flame ionization detector (for CH4) coupled with a methanizer (for CO2) (Agilent Technologies Inc., Santa Clara, CA, USA). Gas flux rates were calculated by the slope of the regression line of a linear regression of the gas concentration against time [27 (link)].
7
Quantifying Fecal SCFA Levels
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The concentration of fecal SCFAs was determined by gas chromatography (GC). A total of 0.15 g of feces was homogenized with 0.6 mL of Milli-Q water and centrifuged at 12,000 rpm for 10 min. The supernatants were filtered through a 0.22 µm nylon filter and acidified to pH 2.0–3.0 by adding HCl. SCFA levels in supernatants were measured by a GC Agilent 7890 (Santa Clara, CA, USA). The amount of major SCFAs in the feces was determined using the equation based on the standard curves derived from serially diluted known concentrations of acetate, propionate, butyrate, and isobutyrate.
8
GC-EAD Analysis of Pheromone-Induced Antennal Response
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The antennal electrophysiological activity of the yeast‐derived pheromone was tested by GC‐EAD. An Agilent 7890 gas chromatograph equipped with a flame ionization detector (FID), an HP‐INNOWax column (30 m × 0.25 mm internal diameter and 0.25 μm film thickness; J&W Scientific, Folsom, CA, USA) was used. Antennae of male S. exigua and P. interpunctella with both tips cut off and associated with the head were mounted on a PRG‐2 EAG (10× gain) probe (Syntech, Kirchzarten, Germany) using conductive gel (Blågel, Cefar, Malmö, Sweden). The antennal preparation was put in a flow of charcoal‐filtered and humidified air that passed through the column outlet. Hydrogen was used as the carrier gas with a constant flow of 1.8 mL min–1, and the GC effluent was directed to the FID and EAD by a 1:1 division. The GC inlet was set at 250 °C, the transfer line was set at 255 °C, and the detector was set at 280 °C. The GC oven was programmed from 80 °C for 1 min, then increased to 210 °C at a rate of 10 °C min–1 and held for 10 min. Data were collected with the software GC‐EAD Pro Version 4.1 (Syntech).
9
Biofluid and Tissue Metabolomics Analysis
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The biofluid metabolomics was examined using an Agilent 7890 gas chromatography system equipped with a Pegasus 4D time of flight mass spectrometer (LECO, St. Joseph, MI, USA) [6 (link)]. The tissue metabolomics procedures were performed as follows. First, 100 mg of MG tissue from each sample was added to a 2-mL Eppendorf tube with 0.4 mL of methanol-chloroform (Vmethanol: Vchloroform = 3:1) and 30 μL of L-2-chlorophenylalanine (1 mg/mL, stored in dH2O) and was mixed by vortexing for 10 s. Second, steel balls were placed in the tube and milled for 5 min at 55 Hz. The sample was then centrifuged at 4 °C at 12,000 rpm for 15 min. Third, approximately 0.4 mL of supernatant was transferred into a 2 mL silylated vial. An equal volume (10 μL) of each sample was placed in a new 2 mL silylated vial as a mixed sample for the quality control of the stability of the equipment system, the standard deviation of the beginning, middle and ending retention time of the mixed samples was less than 0.2, which indicates good stability.
10
Microbial Community Analysis via PLFA
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The method for phospholipid extraction was adapted from Buyer et al.67 (link). Briefly, 3 g of lyophilized soil were placed in a 30-ml centrifuge tube with a Teflon-lined screw cap. The fatty acids were directly extracted from the soil twice by adding 3.6 ml of citrate buffer (pH 4.0), 4 ml of chloroform, and 8 ml of methanol. The PLFAs were separated from neutral and glycolipid fatty acids by solid-phase-extraction chromatography. After mild alkaline methanolysis, the PLFA samples were qualitatively and quantitatively analyzed using an Agilent 7890 gas chromatograph (Agilent Technologies, Santa Clara, USA) equipped with an autosampler, split-splitless injector, and flame ionization detector. The system was controlled with Agilent ChemiStation and MIDI Sherlock software (Microbial ID, Inc., Newark, USA). An external standard of 19:0 methyl ester was used for quantification.
We selected the following PLFA signatures to serve as indicators of specific microbial groups: iso- and anteiso-branched fatty acids for Gram-positive (G+) bacteria68 (link), monounsaturated and cyclopropyl 17:0 and 19:0 fatty acids for Gram-negative (G−) bacteria69 (link), and 18:2w6c for fungi70 (link). Total biomass was obtained by summing the concentrations of all fatty acids detected in each soil sample.
We selected the following PLFA signatures to serve as indicators of specific microbial groups: iso- and anteiso-branched fatty acids for Gram-positive (G+) bacteria68 (link), monounsaturated and cyclopropyl 17:0 and 19:0 fatty acids for Gram-negative (G−) bacteria69 (link), and 18:2w6c for fungi70 (link). Total biomass was obtained by summing the concentrations of all fatty acids detected in each soil sample.
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